The Effects of Gallic Acid on The Acute Necrotizing Pancreatitis in Rats
Transkript
The Effects of Gallic Acid on The Acute Necrotizing Pancreatitis in Rats
The Effects of Gallic Acid on The Acute Necrotizing Pancreatitis in Rats P-09.04.4-042 B. KURALI, D. US ALTAYII, C. KAHRAMANIII, E. ALHANIV, T. MAZLUM SENI, C. ERÇINV, N. KÜÇÜK KENTVI, H. KARAI, S. KÖRI, A. ÖREMI IDepartment of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey, IIUlubey Vocational School- Chemistry and Chemical Processing Technology Department, Ordu University, Ordu, Turkey, IIISchool of Health, Düzce University, Düzce, Turkey, IVDepartment of Surgery, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey, VDepartment of Pathology, Kocaeli University, Kocaeli, Turkey, VISchool of Health, Gümüshane University, Gümüshane, Turkey Reactive oxygen species, playing an active role in the early and late course of acute pancreatitis, lead to dysfunction of cell membrane and releasing of lysosomal enzymes, and thereby to the injury in pancreatic cells. Gallic acid, found in vegetables such as green tea, is an active component which has antioxidant, antiinflammatory, antiviral, anticancer activities. The aim of this study was to investigate the effects of gallic acid in experimental acute necrotizing pancreatitis (ANP) model in rats. Eighteen male Sprague-Dawley rats were divided into three groups (6 rats in each group). Group 1: sham + saline; group 2: ANP induced by intraductal glycodeoxycholic acid and intravenous cerulein; and group 3: ANP + gallic acid (100 mg/kg/day, intraperitoneal). At the end of 18th hours, pancreas histopathology was examined. The levels of serum amylase as a diagnostic marker of pancreatitis, interleukin-6 (IL-6), total antioxidant status (TAS), nitrite + nitrate, total thiols as antioxidant marker and thiobarbituric acid reactive substances (TBARS) to measure malondialdehyde (lipid peroxidation product) were determined by spectrophotometric methods. Serum amylase, IL-6, plasma TBARS levels were significantly higher but total thiols levels were lower than sham group in ANP group without treatment (p < 0.05). However; TAS and nitrite + nitrate levels did not show any significant difference (p>0.05). On the other hand, while serum amylase, IL-6 and TBARS levels were lower, total thiols levels higher in gallic acid treatment group than in the untreated ANP group, but statistically insignificant (p>0.05). In conclusion, gallic acid treatment is beneficial but not sufficient to treat the acute necrotizing pancreatitis in rats.