University Research Laboratories
Transkript
University Research Laboratories
ISTANBUL UNIVERSITY RESEARCH LABORATORIES 2014 İstanbul University Central Campus 34452 Beyazıt / Fatih-İstanbul +90 (212) 440 00 00 iubilgi@istanbul.edu.tr istanbul.edu.tr facebook.com/istanbuluniversitesi1453 twitter.com/istanbuledutr Contents LABORATORY OF INTERACTIONS BETWEEN SUBSTANCE AND MIND 10 NEUROIMMUNOLOGY and IMMUNOGENETICS LABORATORY 11 LABORATORY of COGNITIVE NEUROSCIENCE 12 BIOPHYSICS LABORATORY 13 LABORATORY of ELECTROENCEPHALOGRAPHY 14 GENETIC RESEARCH LABORATORY 15 HISTOLOGY and EMBRYOLOGY RESEARCH LABORATORIES 16 LABORATORY of CHILD HEALTH and DISEASES, and NUTRITION METABOLISM 17 LABORATORY of NEUROPSCHOLOGY 18 MICROBIOLOGY RESEARCH LABORATORIES 19 PEDIATRIC ENDOCRINOLOGY RESEARCH LABORATORY 20 LABORATORY of MEDICAL BIOLOGY 21 LABORATORY of BRAIN and NEURODEGENERATIVE/NEUROGENETIC DISEASES 22 CENTER for HEARING and SPEECH DISORDERS / LABORATORY of AUDIOLOGY 23 SLEEP and WAKE DISORDERS UNIT 24 LABORATORY of PEDIATRIC GENETICS 25 LABORATORY of MOLECULAR GENETICS 26 ANALYTICAL CHEMISTRY RESEARCH LABORATORY 27 PHARMACOGNOSY RESEARCH LABORATORY 28 PHARMACOLOGY RESEARCH LABORATORY 29 PHARMACEUTICAL TECHNOLOGY RESEARCH LABORATORY 30 PHARMACEUTICAL TOXICOLOGY RESEARCH LABORATORY 31 ISTE (HERBARIUM) 32 LABORATORY of ANDROLOGY 33 LABORATORY of DNA ANALYSIS 34 FOSSIL DNA LABORATORY 35 FOOD SCIENCE LABORATORIES 36 CELL CULTURE LABORATORY 37 IN VITRO FERTILIZATION and EMBRYO TRANSFER LABORATORY 38 CARCASS and MEAT QUALITY LABORATORY 39 OSTEOARCHAEOLOGY LABORATORY 40 VIROLOGY RESEARCH LABORATORY 41 FLOW CYTOMETRY LABORATORY 42 RARE DISEASES RESEARCH LABORATORY 43 MOLECULAR HEMATOLOGY RESEARCH LABORATORY 44 TUBERCULOSIS MOLECULAR EPIDEMIOLOGY LABORATORY 45 LABORATORY of TISSUE CULTURE and PROTEOMICS 46 LABORATORY of NEUROIMMUNOLOGY 47 LABORATORY for PRODUCTION, PURIFICATION and INVESTIGATION of EXPERIMENTAL ANIMALS 48 MOLECULAR METABOLIC RESEARCH LABORATORY 49 ISOLATION and TRANSPLANTATION of ISLETS of LANGERHANS RESEARCH LABORATORY 50 HEMATOPOIETIC and STEM CELL LABORATORY 51 HULUSİ BEHÇET LIFE SCIENCES RESEARCH LABORATORY 52 OBSERVATORY IST60 TELESCOPE 56 SOLAR OBSERVATORY 57 CELL and TISSUE CULTURE LABORATORY 58 MOLECULAR CELL BIOLOGY RESEARCH LABORATORY 59 BIOCHEMISTRY RESEARCH LABORATORY 60 PRIMARY-STEM CELL and CANCER RESEARCH LABORATORY 61 RADIOECOLOGY LABORATORY 62 MOLECULAR CANCER BIOLOGY LABORATORY 63 IMMUNOHISTOCHEMISTRY RESEARCH LABORATORY 64 PHYSIOLOGY RESEARCH LABORATORY 65 MOLECULAR RADIOBIOLOGY and BIOMOLECULAR TECHNOLOGY RESEARCH LABORATORY 66 PLANT BIOTECHNOLOGY and GENETICALLY MODIFIED ORGANISMS LABORATORY 67 ENVIRONMENTAL and INSTRUMENTAL ANALYSIS LABORATORY 68 FUNDAMENTAL and INDUSTRIAL MICROBIOLOGY RESEARCH LABORATORY 69 MOLECULAR RADIOBIOLOGY and PLANT BIOTECHNOLOGY RESEARCH LABORATORY 70 PLANKTON CULTURE LABORATORY 71 ISTF (HERBARIUM) 72 NANO and OPTOELECTRONIC RESEARCH LABORATORY 73 ADVANCED LITHOGRAPHIC METHODS LABORATORY 74 HIGH MAGNETIC FIELD and LOW TEMPERATURE LABORATORY 75 MOLECULAR SPECTROSCOPY LABORATORY 76 LASER SPECTROSCOPY LABORATORY 77 DIELECTRIC SPECTROSCOPY LABORATORY 78 NUCLEAR PHYSICS RESEARCH LABORATORY 79 ANCIENT DNA RESEARCH LABORATORY 80 PLANT BIOTECHNOLOGY LABORATORY 81 PROTEOMICS LABORATORY 82 MOLECULAR DIAGNOSIS in HUMAN DISEASES LABORATORY 83 PLANT GENOMIC RESEARCH LABORATORY 84 BIOINFORMATICS LABORATORY 85 GENETICALLY MODIFIED ORGANISMS LABORATORY 86 PLANT PATHOLOGY LABORATORY 87 PLANT SECONDARY METABOLISM ENGINEERING LABORATORY 88 YEAST GENETICS,TECHNOLOGY and GENOMIC RESEARCH LABORATORY 89 WASTE TECHNOLOGY RESEARCH LABORATORY 90 ORGANIC CHEMISTRY RESEARCH LABORATORY 91 FOOD ANTIOXIDANTS MEASUREMENT APPLICATION and RESEARCH CENTER 92 BIOCHEMISTRY RESEARCH LABORATORY 93 PROCESS and REACTOR DESIGN RESEARCH LABORATORY 94 GEOCHRONOLOGICAL SAMPLE PREPARATION and MINERAL SEPARATION LABORATORY 95 SOIL SCIENCE (AGROLOGY) and ECOLOGY LABORATORY 96 LABORATORY and THERMAL ANALYSIS LABORATORY 97 PROF. DR. A. YILMAZ BOZKURT WOODEN MATERIALS COMBUSTION TEST and ANALYSIS LABORATORY 98 ANNUAL RING RESEARCHES and WOOD ANATOMY LABORATORY 99 100 PROF. DR. A. YILMAZ BOZKURT WOOD ANATOMY LABORATORY FORESTRY PRODUCTS CHEMISTRY and TECHNOLOGY RESEARCH LABORATORY and CELLULOSE, PAPER and CARDBOARD ANALYSIS LABORATORY 101 BASIN MANAGEMENT LABORATORY 102 INSTRUMENTAL ANALYSIS LABORATORY 103 ECOTOXICOLOGY and CYANOTOXIN LABORATORY 104 TECHNOLOGY TRANSFER APPLICATION AND RESEARCH CENTER CENTRAL RESEARCH LABORATORY (MERLAB) 105 PRODUCTION and POST-PRODUCTION LABORATORY 108 PREHISTORY LABORATORY 109 SHIP CONSERVATION and RECONSTRUCTION LABORATORY 110 PREFACE İstanbul University is the first university in Turkey and among the first ten universities founded in Europe, directing the society in areas of health, social sciences, natural science and engineering as well as culture and art throughout its 561-year history. It is the biggest and the most established university in Turkey, running 767 diploma programs, with its 20 Faculties, 17 Institutes, 3 Colleges, 1 Conservatoire, 7 Vocational Schools, 41 Application and Research Centers, and 3 Departments. It has a unique training and education potential with its approximately 18,000 graduates. The objectives of İstanbul University, among world’s top 500 universities (ARWU ranking) for the past eight years, for the near future are to be among the world’s top 100 universities and maintain its greatest role in shaping our past/history since 1453 as a “Bridge of Science from the Past into the Future”. Istanbul University has the highest number of publications in Turkey, the number of publications per faculty member increases approximately by 5% each year. It was the serious support attempts for improving the research facilities of scientists at the international level and for strengthening their motivation that led to the attainment of this success. What plays a key role in the progress of nations in the scientific arena is the support given to fundamental scientific research. However, in a globalized world, research that benefits the society is the only way to achieve sustainable development, welfare the whole society enjoys, and a future for a world where we can live. The achievement of this objective is through collaboration of all relevant institute and institutions in areas of research on applied sciences, without neglecting fundamental scientific research. In the last five years, İstanbul University has recognized it as its primary objective to carry its research capacity beyond scientific publications and transform it into new technology and products offering solutions to the problems of our country, region and world society. Our mission is to produce sustainable solutions to the problems in the fields of environment, energy sources, social area, health and culture by research relevant for the society, on the other hand, our vision is to achieve this objective through regional and academic cooperation and in ten years to carry this process to a level that will draw attention of the international academic community. The aim, in preparing the Catalogue of Research Laboratories at İstanbul University, is to enhance our collaboration with our national and international partners in the fields of health, science, engineering, and social sciences by presenting our university’s research inventory and facilities for research to universities, state institutions, private sector establishments, and the public. We present our research inventory as well as vast opportunities of our laboratories to the attention of universities, state institutions, private sector entities and society through our catalogue, The Catalogue of Research Laboratories of İstanbul University, in order to increase our cooperation with our stakeholders in the fields of health, science, engineering and social sciences. Prof. Dr. Yunus SÖYLET RECTOR HEALTH SCIENCES RESEARCH LABORATORIES ISTANBUL FACULTY of MEDICINE LABORATORY OF INTERACTIONS BETWEEN SUBSTANCE AND MIND R 10 esearch on impact of modulated transcranial electrical stimulation on assertiveness, studies on measurement of thresholds of sensation and pain by electrical stimulation, investigation of impact of transcranial electrical stimulation on sensation-pain threshold and sympathetic system, follow-up using neuromonitarization during brain and nervous system surgeries and comparative electrophysiology studies in invertebrates are conducted, and design and construction of devices as well as development of measuring techniques for these experimental studies continue. Research Infrastructure of the Laboratory Modulated transcranial electrical stimulation system, EEG-Neurofeedback system, Visual stimulation Potential/Electroretinography System (8-channel polygraph, Analog/digital converters, Visual stimulus presentation systems). Collaborations and Projects The laboratory took part in the project titled “Electrical Neuronal Oscillations and Cognition” within the scope of the COST program by European Union. A joint project has been conducted with Macedonian Academy of Sciences on EEG-Neurofeedback within the scope of TÜBİTAK’s (Scientific and Technological Research Council of Turkey) Macedonia-Turkey bilateral research support. Laboratory Supervisor and Contact Information Prof. Dr. Sacit Karamürsel +90 212 4142000 - 32507 sacit@istanbul.edu.tr ISTANBUL FACULTY of MEDICINE NEUROIMMUNOLOGY and IMMUNOGENETICS LABORATORY A mong autoimmune diseases, immunologic mechanisms of nervous system diseases are predominantly investigated. Firstly, biological materials of multiple sclerosis, myasthenia gravis and other related disease groups are stored as in a data bank, evaluated and developed as a multidisciplinary cooperation environment. Research is conducted by evaluation of cellular immune response and genetic examinations. Research Infrastructure of the Laboratory Laminar flow cabinet, CO2 incubator, Inverted microscope, Fluorescent microscope, ELISA reading device, Centrifuge, Thermal cycler, Real time thermal cycler (Light thermal cycler), Nanodrop, Horizontal electrophoresis equipment, Gel imaging and documentation device. Collaborations and Projects We run collaborative studies with Heidelberg University (Germany), Mannheim University (Germany) and Petras University (Greece). Laboratory Supervisor and Contact Information Prof. Dr. Güher Saruhan Direskeneli +90 212 4142000 - 32716 gsaruhan@istanbul.edu.tr 11 ISTANBUL FACULTY of MEDICINE LABORATORY of COGNITIVE NEUROSCIENCE B 12 rain structures and mechanisms activated during such cognitive processes as perception, attention, memory and language in human beings by methods of Electroencephalogram (EEG), Event Related Brain Potentials (ERPs) and Navigated Transcranial Magnetic Stimulation (TMS) are investigated. Laboratory’s main field of research is integration of functional MRI (fMRI) or TMS methods with EEG and ERP measurements by using TMS and Magnetic Resonance Imaging (MRI) compatible EEG/ERP systems. Beside basic brain research, neurobiological mechanisms of various dementias and psychiatric diseases, including primarily Alzheimer’s disease and Parkinson’s disease, as well as effects of cardiovascular factors posing a risk for cognition are investigated Research Infrastructure of the Laboratory EEG/ERPs Laboratory (electromagnetic and sound isolation room, 32 and 64-channel digital EEG amplifier, visual/auditory stimulus presentation systems and software, EEG/ERPs analysis software), MR and TMS compatible 32-channel EEG/ERPs System, Navigated Transcranial Magnetic Stimulation (TMS) System. Collaborations and Projects We have collaborations and joint projects with Bremen University (Germany), Munich Ludwig-Maximilian University (Germany), Max Planck Institute for Human Cognitive And Brain Sciences (Leipzig, Germany), Berlin School of Mind and Brain (Germany) and Cuban Neuroscience Institute. We also conduct joint TÜBİTAK (Scientific and Technological Research Council of Turkey) project with Ankara University and joint İSTKA (İstanbul Development Agency) project with İstanbul Şehir University (Turkey) and joint research with Boğaziçi University (Turkey). Laboratory Supervisor and Contact Information Prof. Dr. Tamer Demiralp +90 212 4142000 - 32288 demiralp@istanbul.edu.tr ISTANBUL FACULTY of MEDICINE BIOPHYSICS LABORATORY I mportant studies on cell and tissue cultures, and stem cells have been performed for many years. It has played a pioneering role in production of Hybridoma and monoclonal antibody in our country. Important studies are performed on purification of proteins, nucleic acids (DNA-RNA (mRNA, tRNA)) and any organelles from cells and tissues, cell and tissue-level imaging by molecular methods (immunofluorescence), recombinant protein production by biotechnological methods, development and purification of polyclonal antibodies, protein-protein/ protein-ligand interactions, intracellular protein trafficking, in vitro protein synthesis, description of post-translational modifications (ADP-ribosylation, phosphorylation) and eukaryotic and prokaryotic protein synthesis mechanisms. There are studies on apoptosis, oxidative stress, effects of antioxidants on oxidative stress, mitochondrial damage, C++ metabolisms and ion channels, mesen- chymal stem cell isolation, culture from tissue and organs, hematopoietic stem cells and cancer stem cell isolation and tissue engineering. Research Infrastructure of the Laboratory Cold room, Hot room, Dark room, Laminar flow cabinet, CO2 incubator, Inverted microscope, Fluorescence microscope, Thermal cycler, Semi-dry “Western Blot”, Vertical and horizontal electrophoresis equipment, Lyophilizer, Viscometer, Mixer (vortex), Liquid chromatography purification system, Fluorescence spectrophotometer, UV-Vis spectrophotometer, Transilluminator, Electrophoresis tank. Collaborations and Projects We conduct collaborative studies with MD Anderson Cancer Research Center (USA) and Semmelweis University (Hungary). Laboratory Supervisor and Contact Information Prof. Dr. Işıl Albeniz Prof.Dr. Muhammet Bektaş +90 212 4142000 - 32483 ialbeniz@istanbul.edu.tr +90 212 4142000 - 32487 muhammet@istanbul.edu.tr 13 ISTANBUL FACULTY of MEDICINE LABORATORY of ELECTROENCEPHALOGRAPHY R 14 outine and urgent EEG recording for diagnosis and differential diagnosis of neurological diseases are performed. Hyperventilation and photic stimulation are performed for each patient. Polysomnography and other reflex triggers are used when required. Both short-term and long-term Video-EEG stimulant recordings are used for diagnosis of pseudoseizures and seizure types. Moreover, 24-hour and continuous preoperative monitorization for epilepsy surgery is performed. It is used as a supportive method for such developing and internal diseases as psychiatric disorders and encephalography. Other than diagnostic recordings, we also conduct scientific projects for research purposes. Research Infrastructure of the Laboratory EEG, Video-EEG device and short-term and longterm continuous Video-EEG. Collaborations and Projects Projects and studies are conducted with 31 centers primarily including Germany, Italy and France within the frame of Epicure 6th Framework European Union Integrated Project as well as with Munich Ludwig Maximilian University (Germany), Genoa University (Italy) on genetic epilepsies with the project titled Reflex seizures in different epilepsies, and lastly with Italy and the Netherlands on photosensitive epilepsies within the frame of Marie-Curie Programme. Laboratory Supervisor and Contact Information Prof. Dr. Betül Baykal +90 212 41420000 - 32598 baykanb@istanbul.edu.tr ISTANBUL FACULTY of MEDICINE GENETIC RESEARCH LABORATORY F or postnatal and prenatal diagnosis, chromosome obtainment with short-term and long-term cell cultures, chromosome analysis in different tissues, FISH and a-CGH analyses, DNA isolation, Sanger Sequencing, MLPA real time PCR, enzyme cutting, target mutation in genes, and STR and bioinformatic analyses are performed. Research Infrastructure of the Laboratory Sterile cabinet, Inverted microscope, Hybridization device (Hybridizer), Microcentrifuge, Automatic metaphase finder, FISH microscope with fluorescence attachment, PCR device, Hybridization oven, magnetic mixer, Microarray scanner, Microarray dryer, Genetic Analyzer (Sequencer), Microcentrifuge, Vortex, Plate centrifuge, Nanodrop, Digital dry bath, DNA isolation device. Collaborations and Projects There are collaborations with Cologne University (Germany), University of Paris (France), University of Duisburg Essen (Germany) and Hacettepe University (Turkey) as part of international projects, CRANIRARE and SBAG-ERA-NET. Joint studies are carried out with San Diego University on central nervous system abnormalities and exon sequencing. Laboratory Supervisor and Contact Information Prof. Dr. Seher Başaran +90 212 4142000 - 32327 basarans@istanbul.edu.tr 15 ISTANBUL FACULTY of MEDICINE HISTOLOGY and EMBRYOLOGY RESEARCH LABORATORIES S 16 upplementary analyses for differential diagnosis of nephropathologic diseases and dermatological bullous diseases, primary ciliary dyskinesia and some metabolic neurodegenerative diseases as well as etiopathogenesis of spermatozoa-induced infertility cases and malabsorption syndromes are performed. Preliminary tests for materials used in practices of fields such as dentistry and orthopedics, and primary fibroblast culture procedures are performed. Research Infrastructure of the Laboratory Electron microscope (Jeol 1011), ultramicrotome (Leica EM-UC7), tissue processor (Leica EM-TP), Knifemaker (Leica EM-KMR3), EM-Trim 2, laminar flow cabinet, CO2 incubator, inverted phase contrast microscope, fluorescence microscope and cyto-counter. Collaborations and Projects There are research projects conducted with Leibniz Molecular Pharmacology Institute (Germany), Open University (UK) and Karolinska Institute (Sweden). Laboratory Supervisor and Contact Information Prof. Dr. Seyhun Solakoğlu Prof. Dr. Bülent Ahıshalı Prof. Dr. Leyla Tapul +90 212 4142000 - 32364 seyhun@istanbul.edu.tr +90 212 4142000 - 32360 +90 212 4142000 - 32366 tapul@istanbul.edu.tr ahishali@istanbul.edu.tr ISTANBUL FACULTY of MEDICINE LABORATORY of CHILD HEALTH and DISEASES, and NUTRITION METABOLISM S creening, determination of phenylalanine-tyrosine by HPLC and BH4 challenge tests are performed in Phenylketonuria analysis unit; screening of biotinidase in serum and biotinidase activity, Beutler test, reducing substances in the urine, monosaccharide chromatography, oligosaccharide chromatography, metilmalonic acid determination, nitroprusside test, dinitrophenylhydrazine test, orotic acid determination, sodium hydroxide test, FeCl3 test, ketone determination, and examinations of amino acids and acyl carnitine by tandem mass spectrometry, HPLC analysis of quantitative amino acids and carbohydrate-deficient glycoproteins (CDG), organic acid analyses by gas chromatography-mass spectrometry and enzyme analyses are performed in advanced metabolic tests unit. Research Infrastructure of the Laboratory HPLC systems, amino acid analyzer, tandem mass spectrometry, gas chromatography-mass spectrometry and fume hood systems. Collaborations and Projects We conduct collaborative studies with European Research Network (Switzerland), Center for Disease Prevention and Control (USA), Screening-Labor Hannover (Germany), Heidelberg University (Germany), University of Zurich (Switzerland), University of Nijmegen (Amsterdam), University of Manchester (UK) and University of Newcastle (UK). Laboratory Supervisor and Contact Information Prof. Dr. Ümit D. Türkoğlu Prof. Dr. Gülden Gökçay +90 212 4142000 - 33202 umturk@istanbul.edu.tr +90 212 4142000 - 31439 guldengokcay@gmail.com 17 ISTANBUL FACULTY of MEDICINE LABORATORY of NEUROPSCHOLOGY I t is the first neuropsychology laboratory of Turkey, where neuropsychological evaluation and aphasia evaluation as well as cognitive rehabilitation are conducted. Evaluations are conducted by both traditional neuropsychological batteries and computerized tests. Research Infrastructure of the Laboratory CANTAB and computerized Stroop WCST tests. Collaborations and Projects We have collaborations with Northwestern University (USA) and Rutgers University (USA). 18 Laboratory Supervisor and Contact Information Prof. Dr. Hakan Gürvit +90 212 4142000 - 32574 gurvit@istanbul.edu.tr ISTANBUL FACULTY of MEDICINE MICROBIOLOGY RESEARCH LABORATORIES I t comprises laboratory of bacteriology, laboratory of mycology/mycobacteriology, laboratory of parasitology and laboratories of virology-immunology. Blood culture, sterile body fluids, cystic fibrosis patient samples and interferon-gamma release test in blood samples as well as PCR procedures are performed and Echinococcus granulosus IgG, Entemoeba histolytica IgG are studied. Autoantibodies are examined using ELISA, indirect immune fluorescence and immunoblot method, and immunophenotyping tests and immunodeficiency panel are performed. Research Infrastructure of the Laboratory Ovens operating at various temperatures (incubators), CO2 oven, Normal and refrigerated centrifuges, Normal water bath and water bath with shaker, Elisa devices, Fully automated blood culture device, Fluorescence microscope, Dark field microscope, Automated bacterial identification system, Light cycler (for molecular tests), Thermal cycler to be used in PCR tests, Pulsed Field device (for molecular tests), lyophilizer in culture collection center, Electrophoresis device, Gel imaging system (transilluminator), Biosafety cabinets and flow cytometry. Collaborations and Projects We conduct joint projects with Public Health Agency of Turkey and World Health Organization on infectious diseases and anti-tuberculosis drug resistance. We submit data about activity of flu to National Reference Laboratory for Influenza, Ministry of Health and World Health Organization. We collaborate with University of Cologne (Germany) on diagnosis of HIV drug resistance. Laboratory Supervisor and Contact Information Prof. Dr. Nezahat Gürler +90 212 4142000 - 32377 ngurler@istanbul.edu.tr 19 ISTANBUL FACULTY of MEDICINE PEDIATRIC ENDOCRINOLOGY RESEARCH LABORATORY B lood samples and data from patients diagnosed with congenital hyperinsulinism, neonatal diabetes, obesity, congenital adrenal hyperplasia, thyroid diseases, type 1 diabetes, MODY, multiple pituitary hormone deficiencies, idiopathic shortness of stature, excess aromatase and early puberty are collected. DNA, RNA, leukocyte and serum are extracted and studied. Research Infrastructure of the Laboratory DNA and RNA extraction robot, PCR, Real time PCR. Collaborations and Projects We have collaborations with Exeter University (UK) about congenital hyperinsulinism and neonatal diabetes cases, with Schneider Children’s Medical Center (Israel) about a genetic analysis in early puberty, with University of Prague (Czech Republic) on 20 a genetic study of multiple hormone deficiency and joint studies with TÜBİTAK (Scientific and Technological Research Council of Turkey). Laboratory Supervisor and Contact Information Prof. Dr. Firdevs Baş +90 212 4142000 - 31645 firdevsb@istanbul.edu.tr ISTANBUL FACULTY of MEDICINE LABORATORY of MEDICAL BIOLOGY I t consists of Laboratory of Tissue Typing and Immunogenetics and Laboratories of Serology, Cell Culture, Flow Cytometry, Molecular Genetics, DNA Sequence Analysis and Immunogenetics. Low/high-resolution analyses of HLA tissue groups in patients/donors preparing for transplantation of solid organs and bone marrow/stem cells along with tissue groups considered to be related with autoimmune diseases are studied. Cross-match tests and anti-HLA antibody screening and identification tests which can estimate success of transplantation from living/ cadaveric donors to patients are conducted. Pre-transplantation HLA tissue typing tests and chimerism assay used in post-transplantation engraftment follow-up are conducted on patients/donors planned to have stem cell transplantation. Research Infrastructure of the Laboratory CO2 incubator, Inverted microscope, Fluorescence microscope, Thermal cyclers, Horizontal electrophoresis equipment, DNA sequencer, Flow cytometry device and luminex device. Collaborations and Projects It has been recognized as an accredited laboratory by European Federation for Immunogenetics (EFI). It has been appointed by both EFI Quality Control Committee and Scientific Council for Immunology of Ministry of Health to organize HLA control tests among HLA laboratories. International projects are conducted in the field of Transplantation Immunology/Immunogenetics and Ageing. Laboratory Supervisor and Contact Information Prof. Dr. Fatma Savran Oğuz +90 212 4142000 - 32461 oguzsf@istanbul.edu.tr 21 CERRAHPAŞA FACULTY of MEDICINE LABORATORY of BRAIN and NEURODEGENERATIVE/NEUROGENETIC DISEASES P 22 rimary neuron culture, gene expression analyses, siRNA gene silencing, qrtPCR methods, polymorphism analyses, Protein analyses with Western Blot, analysis of cytokines, chemokines and hormones from body fluids by ELISA method are performed. Roles of different genes in diseases can be determined by neurodegenerative disease models generated using primary neuron culture, and gene silencing methods. The main fields of study are molecular mechanisms involved in neurodegeneration and genetic basis of Alzheimer’s disease and Parkinson’s disease. Research Infrastructure of the Laboratory Laminar flow cabinet in Class 100 Room, CO2 incubator, Inverted microscope, Fluorescence microscope, Thermal cycler, Real time thermal cyclers (Light Cycler 480, Light Cycler Nano), Picodrop, iBlot for Western Blot, Horizontal and vertical electrophoresis equipment, Gel imaging and documentation device. Collaborations and Projects We conduct collaborative studies with Rutgers University (USA), University of Angers (France), University of Exeter (UK) and Thessaloniki School of Medicine of Aristotle University (Greece). Currently a project on discovery of new biomarkers for Alzheimer’s and Parkinson’s diseases, funded by European Union, is conducted. Laboratory Supervisor and Contact Information Prof. Dr. Selma Yılmazer +90 212 4143000 - 22032 syilmazer@istanbul.edu.tr Assoc. Prof. Dr. Duygu Gezen-Ak Assoc. Prof. Dr. Erdinç Dursun +90 212 414 2000 - 21627 dgezen@istanbul.edu.tr +90 212 414 2000 - 21627 erdincdu@istanbul.edu.tr CERRAHPAŞA FACULTY of MEDICINE CENTER for HEARING and SPEECH DISORDERS / LABORATORY of AUDIOLOGY S uch studies as hearing tests for neonates, children and adults, diagnosis and therapy programs for speech-voice and language disorders, scholastic follow-up of and support for individuals with hearing loss, prognosis and rehabilitation of balance disorders, noise and hearing conservation programs, hearing screening programs for neonates, preschool and school-aged children, hearing aid, and implants of middle ear, inner ear and brain stem are conducted. Research Infrastructure of the Laboratory Computerized dynamic posturography, Head impulse, Measurement equipment for cortical auditory evoked potential responses, Multi-frequency immitancemetry, Voice analysis system. Collaborations and Projects We participate in supporting individuals with hearing loss by the joint project with İstanbul Development Agency. We have projects on development of an analysis algorithm for dynamic vestibular system, and medical equipment development within the scope of TÜBİTAK (Scientific and Technological Research Council of Turkey) project conducted in collaboration with İstanbul Technical University (Turkey). Laboratory Supervisor and Contact Information Prof. Dr. Ahmet Ataş +90 212 4143000 - 22686 atas@istanbul.edu.tr 23 CERRAHPAŞA FACULTY of MEDICINE SLEEP and WAKE DISORDERS UNIT D iagnosis and treatment of sleep disorders and polysomnographic examinations for preliminary diagnosis of epilepsy are performed. It is the only unit in Europe accredited accordind to criteria set by European Sleep Research Society and authorized to deliver training and certification programs for specialists from Europe. Research Infrastructure of the Laboratory 3-channel EEG [F4-M1, C4-M1, O4-M1] for fullnight polysomnography, Two-channel EOG, Superficial EMG of chin, Right/left tibialis anterior, and body position sensor, Oro-nasal sensor, Nasal pressure sensor, Thoracic and respiratory movements, ECG, Recording of respiratory sounds, O2 saturation and synchronous video recording. Collaborations and Projects 24 We conduct a randomized, double-blind study in which a comparison of BF2.649 (Pitolisant) and plasebo in two parallel groups in daytime hypersomnia and frequency of weekly cataplexy attacks in narcoleptic patients with cataplexy is made, along with a device development project for detection of cardio-respiratory events during sleep by a device other than polysomnography, that is a multi-center study as part of a European Union project. Laboratory Supervisor and Contact Information Prof. Dr. Derya Karadeniz +90 212 4143000 - 22151 derya.karadeniz@istanbul.edu.tr CERRAHPAŞA FACULTY of MEDICINE LABORATORY of PEDIATRIC GENETICS C hromosome analysis from blood, skin, bone marrow and prenatal samples (fetal blood, amnion fluid and corion villus biopsy) and diagnosis of micro-deletions by fluorescence in situ hybridization are performed. diseases conducted jointly with Université Paris Descartes-Sorbonne Paris Cité, Institut Imagine, Hospital Necker Enfants Malades, France; Department of Genetics; Research on “cilia” disorders conducted jointly with Department of Molecular Medicine and Birth Defects Research Center of University College London (UCL), England; a study on genetic substructure of Cutis laxa group of disorders jointly conducted with Institut für Medizinische Genetik und Research Infrastructure of the Laboratory Humangenetik, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, Berlin, Germany; Research Karyotech PSI Karyotype+FISH analysis system, In- on Noonan group (RASopathies) of disorders joint- cubator, Microscope, Thermo-Highmeter, Refriger- ly conducted with Dr. Martin Zenker Institute of ated centrifuge, CO2 incubator, pH meter, Laminar Human Genetics, University Hospital Magdeburg flow cabinet, Hybridization unit, Spectrophotome- Leipziger, Germany; Research on diseases accompa- ter, Deep freezer. nied by osteoporosis conducted jointly with Pediatric Endocrinology and Metabolic Bone Diseases De- Collaborations and Projects partment of Pediatrics, Helsinki University Hospital, We conduct the following projects with following Finland; arthrogryposis and Cornelia de Lange group partners: Identification of new genes in different of diseases conducted jointly with Department of genetic diseases conducted jointly with Yale Uni- Molecular and Human Genetics, Baylor College of versity (England), Department of Neurosurgery, Medicine, Houston, USA. Neurogenetics Program; genetic studies of skeletal Laboratory Supervisor and Contact Information Prof. Dr. Beyhan Tüysüz +90 212 414 3000 - 21495 beyhan@istanbul.edu.tr 25 CERRAHPAŞA FACULTY of MEDICINE LABORATORY of MOLECULAR GENETICS B eside studies and analyses regarding diagnosis and treatment planning of many diseases, including routine studies on genetics of single gene disorders, infertility tests and genetics of cancer and leukemia, projects pertaining to research-development studies are conducted in laboratory of molecular genetics and cell culture. Research Infrastructure of the Laboratory 37°C incubator, Light cycler 480 real time PCR device, Thermal cycler, Electrophoresis units, Gel imaging device, Centrifuges (refrigerated, vacuum), DNA isolation device, -20°C and -80°C cooling chambers, Microwave, Oven, ABI 3500 sequence analysis device, Light cycler 2.0 Real Time device, Pyro sequence (for specific sequencing), Rotor Gene (real time device) and Laminar Flow cabinet. Collaborations and Projects 26 Ongoing research & development projects supported by TÜBİTAK (Scientific and Technological Research Council of Turkey) are investigation of MiRNAs in human laryngeal cancer, determination of tongue cancer-specific miRNAs and investigation of MiRNAs in patients with delirium. Laboratory Supervisor and Contact Information Prof. Dr. Mustafa Özen +90 212 4143000 - 21468 mozen@istanbul.edu.tr FACULTY of PHARMACY ANALYTICAL CHEMISTRY RESEARCH LABORATORY D evelopment and validation of spectrometric, high performance liquid chromatographic and gas chromatographic methods for drug analyses constituting the basis of research and development, quality control studies, qualitative and quantitative analyses of drug substances and impurities in pharmaceutical preparations and biological materials are conducted and method validation as well as pharmacopoeia analyses are performed in conformity with international guidelines. Research Infrastructure of the Laboratory Spectrophotometer, Spectrofluorimetry, Flow injection system, High performance liquid chromatography (UV, diode-array, fluorescence and electrometric detection, online SPE-LC, UPLC), Gas chromatography (with flame ionization and mass spectrometric detection). Collaborations and Projects Studies are conducted with Istanbul Technical University (Turkey), Yildiz Technical University (Turkey), Bezmialem Foundation University (Turkey), Yeditepe University (Turkey), Anadolu University (Turkey), Pamukkale University (Turkey), Namik Kemal University (Turkey), Dicle University (Turkey), Batman University (Turkey), Munich University (Germany), Barcelona University (Spain), Cairo University (Egypt) and Free University of Brussels (Belgium). These studies are based on development and validation of new analytical techniques and methods. Laboratory Supervisor and Contact Information Prof. Dr. Serap Sağlık Aslan +90 212 4400000 - 13588 ssaglik@istanbul.edu.tr 27 FACULTY of PHARMACY PHARMACOGNOSY RESEARCH LABORATORY I solation and structural determination of compounds isolated from medicinal plants are performed by using chromatographic methods and conducting spectral analyses. Moreover, various biological activities of plant extracts and isolated compounds are investigated. Research Infrastructure of the Laboratory High performance liquid chromatography (with diode-array and fluorescence detection), Medium pressure liquid chromatography, Flash chromatography, chromatotron, Vacuum liquid chromatography, Vacuum speed concentrator, Lyophilizer, Vacuum oven. Collaborations and Projects Collaborative studies are done with Anadolu University (Turkey), Marmara University (Turkey), Uni- 28 versity of Saarland (Germany), University of Paris (France), University of Mostaganem (Algeria), Free University of Berlin (Germany) and Institute of Leibniz (Germany). Studies have been conducted on isolation of active compounds from plant resources and demonstration of activity. Laboratory Supervisor and Contact Information Prof. Dr. Afife Mat +90 212 4400000 - 13400 afifemat@istanbul.edu.tr FACULTY of PHARMACY PHARMACOLOGY RESEARCH LABORATORY P harmacology of gastrointestinal (i.e. experimental ulcer model) and central nervous systems (experimental and genetic epileptic models), pharmacological studies [examination of impacts of P-glycoprotein and transporter proteins of ABC gene family on pharmacokinetics of medicines and determination of levels of medicines in plasma and other body fluids by high pressure liquid chromatography (HPLC)] are performed in addition to examination of activities of medicines and active substances of plant/synthetic origin in such areas as pharmacology of cardiovascular system (experimental hypertension, diabetic and glaucoma models as well as isolated vascular and cardiac preparations obtained from test animals and humans such as aorta, mesenteric artery, renal artery, retinal artery, basilar artery, human intra-mammary artery, human saphenous vein, human radial artery and heart etc.),. Research Infrastructure of the Laboratory Organ bath system, Myograph system, High performance liquid chromatography, Solid and liquid phase extraction systems (with UV and fluorescence detection), Angendorff system, ELISA device and telemetry system. Collaborations and Projects Studies conducted with Ege University (Turkey), Institute for Biological Rhythms and Cancer of University of Paris XI (France), and University of Paris Nord, Inserm UMR-S698 (France). Studies are in the fields of pharmacology of cardiovascular system and pharmacokinetics. Laboratory Supervisor and Contact Information Prof. Dr. Sönmez Uydeş Doğan +90 212 4400000 - 13418 sonmezdo@istanbul.edu.tr 29 FACULTY of PHARMACY PHARMACEUTICAL TECHNOLOGY RESEARCH LABORATORY D 30 rug formulation studies (solid dosage forms, disperse systems, topical and transdermal drug delivery systems, new drug delivery systems (microspheres, vesicular delivery systems, solid lipid nanoparticles, and polymeric micelles), bio-adhesive systems, ocular, pulmonary, nasal and buccal drug delivery systems and radiopharmaceuticals), drug stability studies and targeted (brain, liver, lungs, bone and colon) drug delivery system studies are carried out. ameter. Collaborations and Projects Collaborative studies are conducted with Marmara University (Turkey), London School of Pharmacy (England), University of Bath (England), Cardiff University (England), Trinity College (University of Dublin) (Ireland), University of Pittsburgh (USA) and Innsbruck Medical University (Austria). Lung-targeted drug delivery, transdermal and dermal, pharmaceutical cell culture and radiopharmacy studies have been carried out. Studies regarding development of anti-cancer medicine as part of Euronanomed project have been completed. SANTEZ project conducted as part of a cooperation between Research Infrastructure of the Laboratory Texture analyzer, Dissolution system, Nano-zetasizer, university and industry, and TÜBİTAK (Scientific and Technological Research Council of Turkey) projects continue. Lyophilizer, High performance liquid chromatography (with diode-array and fluorescence detection), Franz diffusion cell system, ATR-FTIR spectrometer, Cell culture laboratory equipment, Tewameter, Corneometer, Skin pH-meter, Sebumeter and mexLaboratory Supervisor and Contact Information Prof. Dr. Yıldız Özsoy Erginer +90 212 4400000 - 13498 yozsoy@istanbul.edu.tr FACULTY of PHARMACY PHARMACEUTICAL TOXICOLOGY RESEARCH LABORATORY I t consists of Molecular & Analytical Toxicology, Genotoxicity and Cell Culture Laboratories, and Pharmacogenetic Diagnosis Unit. Investigation of mechanisms that play a role in toxicological effects of xenobiotics (especially genetic and epigenetic mechanism studies, in vitro and in vivo detection of DNA and chromosomal damages, and oxidative damages, DNA methylation, histone modifications and changes in gene and protein expressions) is performed. Minimum toxicity and maximum therapeutic effect, targeted delivery of especially chronically administered drugs are intended to achieve a narrow therapeutic index in “Individualized Medicine” and determination of differences of gene and proteins involving in metabolism are carried out by coordinated studies with clinical units. Research Infrastructure of the Laboratory Real time thermal cycler (RT-PCR), Thermal cyclers, Electrophoresis and gel visualization systems, Microscope with fluorescence attachment, High performance liquid chromatography (with diode-array and fluorescence detection), Cell culture laboratory equipment. Collaborations and Projects We work in collaboration with Sabancı University (Turkey), and Boğaziçi University (Turkey), Newcastle University (England), University of Würzburg (Germany) and University of Birmingham (England). Two TÜBİTAK (Scientific and Technological Research Council of Turkey) projects have been carried out. Laboratory Supervisor and Contact Information Prof. Dr. Buket Alpertunga +90 212 4400000 - 13565 tunga@istanbul.edu.tr 31 FACULTY of PHARMACY ISTE (HERBARIUM) I 32 STE contains a significant collection of plant species comprising 70% of all plants in Turkey, including primarily medicinal plants, rich in terms of plant samples from Istanbul and Thrace (90%), some of the medically most important plant species (such as Allium, Arum, Asphodeline, Cirsium, Colchicum, Crocus, Cyclamen, Euphorbia, Ferulago, Helichrysum, Hypericum, Iris, Papaver, Rosa, Salvia, Sedum, and Tulipa) as well as more than 115,000 plant samples. All plant samples are scanned and input is stored in a database called “ISTE Information System” and is available on ISTE website for those interested. ISTE contains all endemic monotypic species in flora of Turkey. Research Infrastructure of the Laboratory It contains photographs of more than 150 types of samples and more than 100 photographs of typus. Stereo microscope fitted with a drawing tube and that transmit images into digital media, Chromosome analysis system, Ice microtome (cryostat). Collaborations and Projects We work in collaboration with Anadolu University (Turkey) and Cumhuriyet University (Turkey). Studies for identification and preservation of flora of Turkey have been conducted in these collaborations. European Union and IUCN projects regarding conservation of biodiversity and its sustainable development are performed. It represents Turkey in programs and databases such as “Euro-Med Plant Base” and “Atlas Florae Europaeae”. Istanbul Development Agency project on honey plants and KAMAG projects supported by TÜBİTAK (Scientific and Technological Research Council of Turkey) on identification of geophytes of Turkey and chromosomes are carried out. Laboratory Supervisor and Contact Information Prof. Dr. Emine Akalın +90 212 4400000 - 13578 akaline@istanbul.edu.tr FACULTY of VETERINARY MEDICINE LABORATORY of ANDROLOGY S tudies are conducted on freezing and long-term preservation of semen of farm animals and pets, development of semen freezing techniques as well as discovery of new additives and methods for cryopreservation. Artificial vaginal mucus and a new measurement technique have been developed for determination of potential fertility. Advanced analyses, as well as determination and preservation studies of potential fertility are carried out on frozen semen. This laboratory also functions as a “Reference Laboratory” of the Ministry of Agriculture and prepares reports required for import authorizations of imported semen. Collaborations and Projects A study involving the freezing of semen samples from five sheep breeds and delivery thereof to National Domestic Animal Gene Bank for establishment of a database was conducted in collaboration with the Ministry of Agriculture, TÜBİTAK-MAM GMBE, Ankara University (Turkey), Uludağ University (Turkey), Selçuk University and Middle East Technical University (Turkey). A study for freezing ram semen has been carried out in collaboration with the Ministry of Agriculture, TÜBİTAK-MAM GMBE, Uludağ University, and University of Missouri (USA) and an appropriate freezing protocol has been developed in order to develop a DNA Analysis Laboratory technique and a freezing technique that can be used in TAGEM (General Directorate of Agricultural Re- Research Infrastructure of the Laboratory search) institutes. Semen analysis equipment, Phase contrast microscope (magnification x40 – x10000), Microscope with fluorescence attachment (magnification x40 x1000), Semen freezing system. Laboratory Supervisor and Contact Information Prof. Dr. Kemal Ak Prof. Dr. Alper Baran +90 212 4737070 - 17257 kemalak@istanbul.edu.tr +90 212 4737070 - 17261 peralp@istanbul.edu.tr 33 FACULTY of VETERINARY MEDICINE LABORATORY of DNA ANALYSIS G enomic and mitochondrial DNA analyses concerning pets have been carried out. Gene polymorphisms of pets in particular (Prion, GH, Prolactin, IGF-1, OPN, PIT-1, FASN, SCD, LEP, Milk Protein Genes, agouti genes etc.) are demonstrated both by the sequence analysis method and the PCRRFLP analysis method. Besides, mitochondrial DNA analyses are performed. Research Infrastructure of the Laboratory Thermal cyclers, UV imaging device, Mini and midi Submarine electrophoresis, Vertical electrophoresis, Refrigerated centrifuge and mini centrifuge. Collaborations and Projects We conduct joint studies with European Science Foundation (Austria) on genomic and mitochondrial DNA analyses. We have collaboration protocols with Yıldız Technical University (Turkey) and Ege Univer- 34 sity (Turkey). Laboratory Supervisor and Contact Information Prof. Dr. Kemal Özdem Öztabak +90 212 4737070 - 17126 oztabak@istanbul.edu.tr FACULTY of VETERINARY MEDICINE FOSSIL DNA LABORATORY D NA isolation is conducted on bones of pets and wild animals unearthed in archaeological excavations and their mitochondrial DNAs are compared with those of living animals. Research Infrastructure of the Laboratory Laminar flow cabinet, UV sterilizer, Electrophoresis, Thermal Cyclers. Collaborations and Projects Goat bones unearthed in excavations of Van-Yoncatepe castle were studied. We conduct research on Ancient DNA Extraction Methods in collaboration with University of York (UK). 35 Laboratory Supervisor and Contact Information Prof. Dr. Kemal Özdem Öztabak +90 212 4737070 - 17126 oztabak@istanbul.edu.tr FACULTY of VETERINARY MEDICINE FOOD SCIENCE LABORATORIES F 36 ood Science Laboratories, with over 30 years of experience, conducts national and international studies in the fields of food science and water hygiene in cooperation with industry and public sector, in an enclosed area of approximately 2000 m2. It comprises Food Microbiology Laboratory, Food Chemistry Laboratory, Food Technology Laboratory, Molecular Analysis Laboratories, and Pilot Research Slaughterhouse. Additionally, laboratories support industry and public sector for routine analyses. Research Infrastructure of the Laboratory Collaborations and Projects Various joint studies on food safety, food security and food authenticity are conducted in cooperation with many national and international institutions, such as Ankara University (Turkey), Uludağ University (Turkey), Adnan Menderes University (Turkey), Selçuk University (Turkey), TÜBİTAK MAM (Marmara Research Center), Pendik Veterinary Research Institute of Turkish Ministry of Food, Agriculture and Livestock, European Union Joint Research Centre, University of Vienna (Austria), Ludwig-Maximilians University Munich (Germany), University of Bristol (England), University of Surrey (England), University of Thessaloniki (Greece), Joseph Fourier University (France), University of Georgia (USA), Texas A&M University (USA), United States Department of Agriculture, Texas Agricultural Research Center (USA). Cooled and regular centrifuges, magnetic and regular mixers and shakers, Ultra centrifuge, Spectrophotometer, Evaporator, PCR thermal cyclers, Gel electrophoresis, Gel screening, Laminar flows, Bio-safety cabinets, Homogenizers, ELISA reader, Automated milk analyzer, MAP, vacuum and stretch packaging machines, Gas analyzer, Texture analyzer and its apparatus, chromometer, Incubators, Stomacher, Blender and smashers, Aw-meter, Soxhlet, Trichinoscope, analysis equipment and kits for food microbiology and food technology. Laboratory Supervisor and Contact Information Prof. Dr. Gürhan Çiftcioglu +90 212 4737070 - 17222 gurhan@istanbul.edu.tr FACULTY of VETERINARY MEDICINE CELL CULTURE LABORATORY P harmacological effects of new drug molecules or candidate drug molecules on healthy and cancer cell progenies are analyzed by molecular techniques for elucidation of mechanisms of action thereof. Moreover, we conduct molecular studies on determination of pharmacological effects of various plant extracts, which are raw materials of drugs. Potential genotoxic effects of compounds are determined using various genotoxicity tests (comet and micronucleus test). Collaborations and Projects We conduct research as part of TÜBİTAK (Scientific and Technological Research Council of Turkey) and COST projects. Studies such as molecular investigation of pharmacological effects of drug substances on cancer cells, determination of pharmacological properties and biological activities of plant extracts, investigation of effect of a commonly used veterinary antibiotic on anaerobic digestion systems producing biogas from animal manure and determination of outcome thereof in receiver environment, increasing biomethane productivity in anaerobic digesters by Research Infrastructure of the Laboratory Multi-mode Reader System for Absorbance, Fluores- using specific rumen bacteria and hydrolysis enzymes are performed in collaboration with different departments of various universities. cence and luminescence measurements, Cell counter, CO2 incubator, Western Blot, Thermal Cycler, Inverted Microscope, Fluorescence microscope, Lyophilizer, Spectrophotometer with Dual Beam and Xenon Lamp, Class II Biological safety Cabinet, protein detection device, Vertical and horizontal gel electrophoresis, Ultrapure Water system. Laboratory Supervisor and Contact Information Prof. Dr. Tülay Bakırel +90 212 4737070 - 17141 tulbakir@istanbul.edu.tr 37 FACULTY of VETERINARY MEDICINE IN VITRO FERTILIZATION and EMBRYO TRANSFER LABORATORY I 38 n 2000, lambs, the first farm animals to be produced by in vitro fertilization in Turkey, were born in this laboratory, in 2007 Turkey’s first animals were cloned, in 2009 the first grey bull was cloned, and in 2010 the first grey female calves were cloned, in 2013 the first lambs were born by intracytoplasmic sperm injection method, the first transgenic rabbits and the first transgenic lambs were born. Development of estrus synchronization techniques in farm animals, oocyte yield in farm animals, pets and laboratory animals, improvement of in vitro culture environments, freezing and long-term preservation of oocytes and embryos, improvement of cloning techniques in cats and sheep, embryonic stem cells, development of gene delivery systems to produce transgenic animals, ICSI transgenesis, sperm-mediated transgenesis and pronuclear gene injection are intensely studied. Research Infrastructure of the Laboratory Inverted microscope with fluorescence attachment, Micromanipulation system, Piezo microinjection, Micromanipulation system, Cell electrofusion system and oxygen and CO2 incubators. Collaborations and Projects Embryos of five sheep breeds were frozen and delivered to National Domestic Animal Gene Bank, by which farm animal species specific to Anatolia are to be protected, that was established in cooperation with the Ministry of Agriculture, TÜBİTAK-MAM GMBE, Ankara University (Turkey), Uludağ University (Turkey), Selçuk University (Turkey) and Middle East Technical University (Turkey). Embryo production studies by ICSI method, using lyophilized semen and mouse embryonic stem cell transfection studies continue in collaboration with Uludağ University (Turkey) and Fatih University (Turkey), respectively. Collaborations with University of Hawaii and mouse embryonic stem cell transfection studies jointly conducted with Fatih University continue. Research was conducted on control of reproduction and biology of reproduction, and a new method was developed for estrus synchronization as a consequence of the projects conducted. Moreover, a vaginal tablet which may be used for estrus synchronization was developed and patent applications were made to TPI (Turkish Patent Institute) and EPO. Laboratory Supervisor and Contact Information Prof. Dr. Serhat Pabuccuoğlu Prof. Dr. Sema Birler +90 212 4737070 -17258 serpab@istanbul.edu.tr +90 212 4737070 - 17259 sbirler@istanbul.edu.tr FACULTY of VETERINARY MEDICINE CARCASS and MEAT QUALITY LABORATORY C haracteristics of meat such as color, tenderness and juiciness are analyzed by reliable scientific methods. Meat samples are evaluated in terms of parameters such as texture analysis, meat and carcass color analysis, passive water loss, water-holding capacity, measurement of loss on cooking, and meat taste panel. Furthermore, studies on determination of quality of carcass (measurement of carcass size, determination of muscle area and composition (meat, bone, fat ratio)) are performed. Research Infrastructure of the Laboratory Texture analyzer and a Warner-Blatzler blade compatible with this device, Chromometer. Collaborations and Projects Joint studies were carried out by researchers from Uludağ University (Turkey), Kafkas University (Turkey), Çanakkale Onsekiz Mart University (Turkey), Mustafa Kemal University (Turkey), and Ondokuz Mayıs University (Turkey) to determine carcass and meat quality traits of farm animals of various species in different production systems used in Turkey, which were supported by TÜBİTAK (Scientific and Technological Research Council of Turkey) and the Ministry of Agriculture. Joint studies were performed with European Federation of Zootechny (Italy) on high quality meat production. Laboratory Supervisor and Contact Information Prof. Dr. Bülent Ekiz Dr. Hülya Yalçıntan +90 212 4737070 - 17230 bekiz@istanbul.edu.tr +90 212 4737070 - 17133 hyalcint@istanbul.edu.tr 39 FACULTY of VETERINARY MEDICINE OSTEOARCHAEOLOGY LABORATORY A rchaeological materials collected from archaeological excavations are analyzed. Identification of species, modifications, and pathologies and all traces and remains are analyzed and answers are sought for many questions including, in particular, those about human-animal relationships in communities in the past. Research Infrastructure of the Laboratory Reference collections. Collaborations and Projects National and international collaborations are established through proposal of scientific projects on analysis of materials from Subway and Marmaray (Yenikapı) excavation, and excavation studies of Kuşadası-Kadıkalesi (Anaia), Amasya-Oluz Mound, Van Castle Mound and Yoros Castle. Excavation 40 analyses are performed in cooperation with Presidencies of Excavations and Archaeology Museums. Within the frame of cooperation with University of Wroclaw (Poland), University of Trakia (Bulgaria) and Academy of Natural Sciences (Belgium), not only projects are carried out, but also joint studies are conducted with foreign scientists. Laboratory Supervisor and Contact Information Prof. Dr. Vedat Onar +90 212 4737070 - 17225 onar@istanbul.edu.tr FACULTY of VETERINARY MEDICINE VIROLOGY RESEARCH LABORATORY S tudies of serological diagnosis of viral diseases and viral zoonoses in animals, virus production methods, and molecular diagnosis and gene analysis are conducted. Moreover, studies on methods of protection from, and control of and eradication of viral diseases are performed. Collaborations and Projects We conduct collaborative studies with Kansas State College of Veterinary Medicine (USA), Liverpool School of Veterinary Medicine (UK), Bristol School of Veterinary Medicine (UK), Ohio College of Veterinary Medicine (USA), Madrid Faculty of Veterinary Medicine (Spain), Hungarian Medical School (Hungary), Bari Faculty of Veterinary Medicine (Italy), Athens Faculty of Veterinary Medicine (Greece), Nippon Institute of Technology (Japan), University of Queensland (Australia), Veterinary Institute of Wageningen (Netherlands), Friedrich Loeffler Institute (Germany). Our projects on detection of Hepatitis A and B in environmental samples and water Research Infrastructure of the Laboratory CO2 incubator, Inverted microscope, Shaking incubator, Egg incubator, Refrigerated and normal microcentrifuges, Ultra-centrifuge, Spectrophotometer, samples as part of TÜBİTAK (Scientific and Technological Research Council of Turkey) projects and investigation of infectious bronchitis virus in poultry as part of COST project still continue. Real time PCR devices, Gel electrophoresis, Gel imaging device, Bio II safety cabinets, Homogenizers, Thermoblocks, Western blot, ELISA reader, Lyophlizer. Laboratory Supervisor and Contact Information Prof. Dr. Hüseyin Yılmaz +90 212 4737070 - 17069 hyilmaz@istanbul.edu.tr 41 INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH FLOW CYTOMETRY LABORATORY P resence and functions of immune system cells are determined and proteins presented on the cell surface or inside the cell and their cell functions are demonstrated by fluorescent-labelled mono-clonal antibodies, and specific cell populations can be purified. Collaborations and Projects We have collaboration protocols with University of Giessen (Germany), Allergy and Asthma Research Center (Switzerland), St. Anna Children’s Hospital (Austria), Uludağ University (Turkey), Medipol University (Turkey), Marmara University (Turkey), Bezm-i Âlem Foundation University (Turkey), Kocaeli University (Turkey), Ankara University (Turkey), Ege University (Turkey), Selçuk University (Turkey), and Yüzüncüyıl University (Turkey). 42 Mechanisms involved in immunopathogenesis of Behçet’s disease, Multiple Sclerosis, and allergy and asthma are investigated as part of TÜBİTAK (Scientific and Technological Research Council of Turkey) Research Infrastructure of the Laboratory FACSCalibur Flow Cytometry (4 color analysis projects and projects are conducted on development of new technologies for treatment of Type 1 Diabetes as part of the Inten-C project. of subcellular groups stained by 4 different color fluorescent-labelled antibodies at the same time), FACSAria II Flow Cytometry (10 color analysis of cells at the same time), Refrigerated centrifuge, Laminar flow cabinet. Laboratory Supervisor and Contact Information Prof. Dr. Günnur Deniz +90 212 4142000 - 33306 gdeniz@istanbul.edu.tr INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH RARE DISEASES RESEARCH LABORATORY R esearch techniques required for investigation and diagnosis of rare diseases and cerebral conditions such as epilepsy, ataxia etc., including undiagnosed diseases frequently occurring in Turkey as a consequence of consanguineous marriages and tradition of having more than one child, are developed and applied in our laboratory. We study individual cases with multiple congenital anomalies and/or mental retardation conditions by SNP-array based molecular karyotyping approach. In familial studies, data obtained from such new generation techniques as whole genome methylation and whole genome exome sequencing based on SNP genotyping, whole genome linkage and haplotype analyses are analyzed. We have currently been constructing a DNA bank, a rich inventory to be used as reference for individuals with rare genetic neurological diseases. Rare Diseases Research Laboratory is Orphanet’s coordinator in Turkey. Research Infrastructure of the Laboratory 454 GS FLX Sequencer, GS Junior System, Confocal microscope. Collaborations and Projects A DNA bank was constructed by collecting DNA samples from epilepsy patients as part of an EU-funded FP6 integrated project on epilepsy (EPICURE) and clinical characterizations of patients were jointly conducted by specialist neurologists from İstanbul Faculty of Medicine and Cerrahpaşa Faculty of Medicine of İstanbul University (Turkey). International Inten-C project is conducted under Turkish (DETAE (Institute of Experimental Medical Research), Boğaziçi University and Sabancı University) and German (Cologne Center for Genomics) partnership, and studies are performed as part of TÜBİTAK (Scientific and Technological Research Council of Turkey) projects. Laboratory Supervisor and Contact Information Prof. Dr. Uğur Özbek +90 212 4142000 - 33312 uozbek@istanbul.edu.tr Assoc. Prof. Dr. Sibel Uğur İşeri +90 212 4142000 - 33312 sibel.ugur@istanbul.edu.tr 43 INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH MOLECULAR HEMATOLOGY RESEARCH LABORATORY W 44 e conduct studies on investigation of signaling pathways involved in the development of leukemia and genes with a prognostic significance in acute leukemia using whole genome expression and methylation array analyses and amplicon deep sequencing applications, as well as frequently occurring primary immune deficiency diseases due to high rates of consanguineous marriages in Turkey. It is the first and only center offering diagnostic services for immune deficiency diseases such as SCID, CVID and XLA in Turkey and conducts gene therapy trials in XLA mouse models. It is also a member of European Leukemia Network (ELN) and the sole IS-certified reference laboratory in Turkey for detection of BCR/ABL. Research Infrastructure of the Laboratory 454 GS FLX Sequencer, GS Junior system, High security cell culture laboratory and cabinets, Bacteria culture laboratory, Confocal microscope and fluorescence microscope and microplate luminescence reader. Collaborations and Projects We perform studies as part of IRON II and IRON III projects under European Leukemia Network and conduct deep sequencing analyses with Roche Life Sciences. Interdisciplinary projects supported by TÜBİTAK (Scientific and Technological Research Council of Turkey) and Turkish Society of Hematology Research Fund are conducted with universities such as İstanbul Faculty of Medicine (Turkey), Cerrahpaşa Faculty of Medicine (Turkey), Marmara University (Turkey), Hacettepe University (Turkey), Ege University (Turkey), Kocaeli University (Turkey). Furthermore, we perform joint projects with Erasmus Faculty of Medicine (The Netherlands), Heidelberg University (Germany) and St Jude Children’s Research Hospital (USA). Laboratory Supervisor and Contact Information Prof. Dr. Uğur Özbek +90 212 4142000 - 33312 uozbek@istanbul.edu.tr Assoc. Prof. Dr. Müge Sayitoğlu +90 212 4142000 - 33312 mugeay@istanbul.edu.tr INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH TUBERCULOSIS MOLECULAR EPIDEMIOLOGY LABORATORY W e conduct studies on detection of DNA fingerprints of strains causing tuberculosis in İstanbul and surrounding areas by molecular methods, and determination of infection dynamics of the region along with research on molecular mycobacteriology. IP 10 levels in whole blood samples stimulated with ESAT 6 and CFP 10 antigens were determined and a IG(P) RA test identifying latent TB was developed. A new species of mycobacteria, “Mycobacterium celeriflavum sp. nov”, has been identified and studies for developing kits for detection of rapid resistance to drugs and identification at the same time continue. It serves as the “Top-level Laboratory of İstanbul Anti-tuberculosis Association” upon a protocol signed between İstanbul Anti-tuberculosis Association and Rectorate of İstanbul University. Research Infrastructure of the Laboratory Light/fluorescence microscope and capillary electrophoresis and sequence analyzer used in DNA fragment analysis and sequence analysis. Collaborations and Projects We conduct joint projects on identification of new species of mycobacteria with San Raffaele Scientific Institute (Italy), detection of Beijing genotype tuberculosis strains in our country and adaptation thereof to regional human populations with St. Petersburg Pasteur Institute (Russia), and genotypic diversity of Mycobacterium caprae and characteristics of the disease it causes in human beings with Agency for Food and Health Safety (Austria). Laboratory Supervisor and Contact Information Assoc. Prof. Dr. O. Kaya Köksalan +90 212 4142000 - 33345 okoksalan@istanbul.edu.tr 45 INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH LABORATORY of TISSUE CULTURE and PROTEOMICS W 46 e conduct various research projects and protein analysis studies in cytogenetic, biochemical and molecular fields using cell culture and tissue culture technology. We examine alterations in gene and protein expression levels, cell life cycle and signaling pathways occurring in parallel with alterations in cells and tissues in different cancer types and such various metabolic disorders as obesity and diabetes, in addition to conducting miRNA and protein microarray analyses. Moreover, we investigate effects of many different substances on etiology and prognosis of diseases. We conduct studies to determine contents, elucidate functions and establish structural properties of proteins in various biological materials. Research Infrastructure of the Laboratory Fluorescence Microscope, Optical microscope, CO2 incubator, Laminar flow cabinet, Particle counter, Cell viability measurement device, Fluorometer, Western Blot device and its equipment. Collaborations and Projects We conduct national and international collaborative studies with University of De Montfort (UK), Sabancı University (Turkey), and İstanbul Technical University (Turkey). In addition, we carry out multiple interdisciplinary projects funded by State Project Organization, İstanbul Development Agency and TÜBİTAK. We carry out joint projects with various organizations and institutions such as BAL-DER which is active in various industrial fields. Laboratory Supervisor and Contact Information Prof. Dr. Oğuz Öztürk +90 212 4142000 - 33303 oguzozturk@istanbul.edu.tr INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH LABORATORY of NEUROIMMUNOLOGY W e conduct studies for elucidation of pathogenesis and identification of new biomarkers for multiple Sclerosis, neuromyelitis optica, autoimmune encephalitis and neuro-Behçet’s diseases using cell culture and molecular biology methods. Various treatment methods, including mesenchymal stem cell application, are tried using immunization-based experimental animal model of myasthenia gravis disease. We examine gene and protein expression levels in tissue samples from different autoimmune neurological diseases and alterations in survival/apoptotic signaling pathways. We conduct studies for identification of new autoantibodies in autoimmune neurological diseases by primary neuronal culture cells, transfected cells and protein macro-array methods. Research Infrastructure of the Laboratory Optical microscope, Laminar flow cabinet, Western Blot, Immunohistochemistry and ELISA device. Collaborations and Projects We conduct various collaborative studies such as experimental myasthenia gravis studies with University of Texas (USA), analysis of intestinal microbiome in neuro-Behçet’s disease with Baylor College of Medicine (USA) and identification of new autoantibodies in neuromyelitis optica cases with University of Patras (Greece). Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Erdem Tüzün +90 212 4142000 - 33356 erdemtuzun@istanbul.edu.tr 47 INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH LABORATORY for PRODUCTION, PURIFICATION and INVESTIGATION of EXPERIMENTAL ANIMALS W e conduct various projects in areas such as housing, care and production of laboratory animals as well as performance of investigational trials thereon and providing subsequent post-operative care thereof. Research Infrastructure of the Laboratory Individually ventilated cage system, Surgical microscope, Microscope imaging system and recorder, infusion pump, Pressurized washing machine (90 L capacity), Optical microscopes, Blood collection containers (for rats, mice, and rabbits), Isolator. Collaborations and Projects We conduct projects in cooperation with Leibniz Institute for Molecular Pharmacology (Germany) and 48 Karolinska Institute (Switzerland) and participate in a COST project on gene transfer in rabbits. In addition, we conduct a TÜBİTAK (Scientific and Technological Research Council of Turkey) project on investigation of neuroprotective properties of curcumin coated with star block-copolymer. Laboratory Supervisor and Contact Information Asst. Prof. Dr. Mutlu Küçük +90 212 4400000 - 33372 m.kucuk@istanbul.edu.tr INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH MOLECULAR METABOLIC RESEARCH LABORATORY V arious biological molecules and metabolites are analyzed to investigate effects of etiopathogenesis of multifactorial diseases and individual and/or environmental variations on metabolism. Research Infrastructure of the Laboratory Real time PCR, Western Blot device and its equipment, Luminex, Atomic absorption device, ELISA reader, ELISA washer, Chemiluminescence. Collaborations and Projects We work in cooperation with Casablanca Hassan II University (Morocco), İstanbul Faculty of Medicine (Turkey), Cerrahpaşa Faculty of Medicine (Turkey), İstanbul University Faculty of Dentistry (Turkey), Bezm-i Alem University (Turkey), Fatih University (Turkey), İstanbul Technical University (Turkey), Uludağ University (Turkey) and many other universities. Furthermore, interdisciplinary projects supported by TÜBİTAK (Scientific and Technological Research Council of Turkey) are under way. Our collaborative research, as part of Technocity project with Uludağ University, on developing a combined kit to help perform personalized chemotherapy for cancer patients continues. Laboratory Supervisor and Contact Information Prof. Dr. İlhan Yaylım +90 212 4142000 – 33329 iyaylim@istanbul.edu.tr 49 INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH ISOLATION and TRANSPLANTATION of ISLETS of LANGERHANS RESEARCH LABORATORY I t has pioneered in experimental isolation and transplantation of islets from experimental animals since 1990s and is the first institution in Turkey to perform isolations of islets of Langerhans from human pancreases, and has laid the foundation for performance of transplantation in the clinic. Research Infrastructure of the Laboratory Class 100,000/10,000 laminar flow sterile room at the size of 50 m2 and positive pressure, Class II safety cabinet, Pancreas digestion chamber (Ricordi’s Chamber), Enzyme perfusion device, Peristaltic pump, Dissection microscope, Islet purification device. Collaborations and Projects We continue our studies with University of Giessen 50 (Germany) on regenerative treatment of Type 1 diabetes and development of new technologies for pancreatic beta cell proliferation as part of a TÜBİTAK (Scientific and Technological Research Council of Turkey) INTEN-C project. Laboratory Supervisor and Contact Information Prof. Dr. M. Temel Yılmaz +90 212 4142000 - 33370 mtemelyilmaz@istanbul.edu.tr INSTITUTE of EXPERIMENTAL MEDICAL RESEARCH HEMATOPOIETIC and STEM CELL LABORATORY W e conduct projects to gain understanding of altered structure, of character and of functions of hematopoietic stem cells that convert into cancer stem cells due to various mutations. Hematopoietic stem cell and cancer stem cell, role of endothelial progenitor cells and endothelial cells in thrombosis, myeloproliferative diseases from hematological malignities and frequently occurring mutations are investigated. Research Infrastructure of the Laboratory Coagulometer, Laminar flow cabinet, Cell culture laboratory, Micro-centrifuge, PCR device. Collaborations and Projects We continue joint projects with Mount Sinai School of Medicine (USA) and Academy of Sciences (Bulgaria). We currently conduct a TÜBİTAK (Scientific and Technological Research Council of Turkey) project that involves investigation of characterization and of functional analysis of cancer-initiating cells in myeloproliferative diseases, and a COST project that involves “examination of effects of JAK2V617F mutation on endothelial cell functions”. Laboratory Supervisor and Contact Information Asst. Prof. Dr. Selçuk Sözer Tokdemir +90 212 4142000 - 33317 ssozer@istanbul.edu.tr 51 HULUSİ BEHÇET LIFE SCIENCES RESEARCH LABORATORY H 52 UBAL is a multidisciplinary, central research laboratory with a great infrastructure and required instruments for life sciences research, established at İstanbul University as part of a project supported by Ministry of Development of Turkey. Units active in the fields of Genomics, Neuroscience, Biotechnology, and Drug Research/ Pharmaceutics have been completed in 2014 and many projects have been initiated in these fields. We aim to contribute to translational research in healthcare with the infrastructure established. HUBAL has been structured so as to make its equipment park accessible not only for researchers from İstanbul University, but also from other universities and research institutions. Research Infrastructure of the Laboratory 3.0 Tesla Magnetic Resonance Imaging (MRI) System, Functional MR (fMRI) Attachment (visual, auditory stimulus presentation system and response buttons), MR Compatible Digital EEG System, Laser Scanning Confocal Microscope, Ultracentrifuge, Real-time Polymerase Chain Reactor, Piezo Impact Micromanipulator, Vertical Air Flow Cabinet, Liquid Nitrogen Storage System, CO2 Incubator, Individually Ventilated Plexiglas Animal Cages. Collaborations and Projects We conduct projects, one of which is supported by EU (JPND), two of which are supported by COST and two of which are supported by TÜBİTAK (Scientific and Technological Research Council of Turkey) ARDEB, in collaboration with Bezmialem University (Turkey), Bilgi University (Turkey), Marmara University (Turkey) and Boğaziçi University (Turkey). We have initiated cooperation with Max Planck Institute (Germany), Berlin Brain and Mind Institute (Germany) and Cuban Neuroscience Center (Cuba). A protocol has been signed between Drug Research/ Pharmaceutics Unit and Novartis as part of University-Industry Cooperation. We work in coordination with Life Sciences and Technologies Application and Research Center of Boğaziçi University. Laboratory Supervisor and Contact Information Prof. Dr. Zeynep Çiğdem Kayacan +90 212 4400000 - 10735 cigdemb@istanbul.edu.tr 53 NATURAL SCIENCES and ENGINEERING RESEARCH LABORATORIES FACULTY of SCIENCE OBSERVATORY IST60 TELESCOPE W 56 e perform photometric observations of binary stars, asteroids and planets orbiting other stars (exoplanets) and determination of both positions and orbital elements thereof. This observatory contributes to international campaign observations organized for identification of planets that are in exoplanetary systems, but cannot be identified by direct observations. Furthermore, it also contributes to projects aimed to discover asteroids that may pose a threat to the Earth and determine whether there are any changes in their orbits compared to previous ones. In addition, we follow changes in orbital periods of binary star systems which account for a significant part of our astrophysical knowledge. Research Infrastructure of the Laboratory A Ritchey-Chrétien optical telescope with a main mirror of 60 cm aperture, Alta U42 model CCD camera with E2V series (CCD42-10) chip, 13.5 x 13.5 microns 2048 x 2048 array pixels, 9-slot filter wheel, Bessel UBVRI and Strömgren uvby-Hβ filter sets, meteorology station. Collaborations and Projects This observatory was founded on premises of Çanakkale Onsekiz Mart University Astrophysics Research Center and Ulupınar Observatory (Turkey) by a protocol based on shared observation time using our university’s resources. Additionally, it contributes to TÜBİTAK (Scientific and Technological Research Council of Turkey) projects. A 5-year research protocol encompassing subjects such as extra-galactic astronomy, cosmology, analyses of images and astronomical catalogues and stellar astrophysics has been signed with Cote d’Azur Observatory (France) as of June 2014. Laboratory Supervisor and Contact Information Prof. Dr. Adnan Ökten Prof. Dr. A. Talat Saygaç +90 212 4440000 - 10295 aokten@istanbul.edu.tr +90 212 4440000 - 10282 saygac@istanbul.edu.tr FACULTY of SCIENCE SOLAR OBSERVATORY S olar observations are made on a daily basis using a mechanism of two separate telescopes attached to an astrograph which forms the main body. The solar activity is monitored based on sunspots and solar flares. One of the observation mechanisms is used to draw the projection disc of the photosphere (light sphere). Second mechanism is used for Hα CCD imaging and monitoring of chromosphere (color sphere). Obtained data are archived both digitally and physically. Research Infrastructure of the Laboratory Astrograph with a 30 cm aperture and 150 cm focal length, Photospheric telescope with a 13 cm aperture and 200 cm focal length, Chromospheric telescope with a 12 cm aperture and 232 cm focal length, Interference filter with a wavelength of 6562.8 Å and a bandwidth of 0.3 Å, A flip-mirror system and an eyepiece, An SBIG-STL series (11000M) astronomical CCD camera with a 4008 x 2672 pixel chip, Meteorology station. 57 Collaborations and Projects Photospheric data obtained are shared with Solar Influences Data Analysis Center (Belgium). We have initiated a 5-year research protocol with Instituto Ricerche Solari Locarno (Switzerland) as of March 2014 covering solar physics subjects. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Nurol Al Asst. Prof. Dr. Mevlânâ Başal +90 212 4440000 - 10288 al@istanbul.edu.tr +90 212 4440000 - 10283 mbasal@istanbul.edu.tr FACULTY of SCIENCE CELL and TISSUE CULTURE LABORATORY E 58 ffects of environmental factors on male reproductive system and development are examined in vitro. Various studies are conducted on development and health of male reproductive system. For instance, agonist and antagonist impacts of substances as arsenic and flouride consumed via diet on reproductive cells. The metabolic products of substances are investigated on receptor binding levels as well as hormone inhibition and induction pathways. Studies conducted in this laboratory with cell lines such as TM3 (Leydig) and TM4 (Sertoli) of male reproductive system reveal that environmental factors affect the metabolic pathways during development of male reproductive system. Moreover, embryonic development of various living organisms is examined. Research Infrastructure of the Laboratory Laminar flow cabinet, Fluorescence inverted microscope and imaging system, Deep freezer (-80°C), Ultrasonicator, Multi-scan spectrum, Homogenizer. Collaborations and Projects We conduct collaborations and projects on xenobiotics, infertility and production of mature sperm from stem cells with Çanakkale Onsekiz Mart University (Turkey), Yeni Yüzyıl University (Turkey), Technical University of Dresden (Germany), University of Porto (Portugal) and Joint Research Centre of Institute for Health and Consumer Protection (Italy). Laboratory Supervisor and Contact Information Prof. Dr. Melike Erkan +90 212 4555700 - 15021 merkan@istanbul.edu.tr FACULTY OF SCIENCE MOLECULAR CELL BIOLOGY RESEARCH LABORATORY W e investigate cell death, damage and repair mechanisms in various in vivo and in vitro experimental disease models and human diseases at a molecular level. Molecular studies are conducted on apoptosis, necrosis, autophagy, cell growth, tissue regeneration, fibrosis, various growth factors and cytokines-related signaling pathways, glycobiology: lectine-carbohydrate interactions and anti-cancer activities of some molecules. DNA and RNA isolation, analysis and imaging, gene expression analyses: RT-PCR, in-situ hybridization, various glycan determination and analysis methods, single and multiple immunohistochemical and immunofluorescence methods, immunoprecipitation, 1D and 2D protein electrophoresis and western blotting methods are applied. Research Infrastructure of the Laboratory Laminar flow cabinet, Ultrasonic water bath, PCR system, Fluorimeter, Refrigerated centrifuge, Homogenizer, Vertical and horizontal electrophoresis systems, IEF system, Gel imaging device, Inverted microscope with fluorescence attachment. Collaborations and Projects We conduct collaborative studies with İstanbul Technical University (Turkey), Marmara University (Turkey), Helmholtz Zentrum München - German Research (Germany), Universidad Nacional Autonoma de Mexico (Mexico), and University of Maryland (USA). TÜBİTAK (Scientific and Technological Research Council of Turkey) funded research and development projects are being conducted. Laboratory Supervisor and Contact Information Prof. Dr. Şehnaz Bolkent +90 212 4555700 - 15079 sbolkent@istanbul.edu.tr 59 FACULTY of SCIENCE BIOCHEMISTRY RESEARCH LABORATORY C 60 ell death, damage and tissue repair mechanisms are investigated biochemically. Also, biological activities of new molecules are examined therapeutically in various experimental disease models and human diseases. Protein isolation and purification, protein assays, determination of enzyme activities, oxidant/antioxidant parameters, apoptotic and necrotic markers, inflammatory and anti-inflammatory agents, and various growth factors and cytokines are analyzed by ELISA, spectrophotometric, colorimetric and electrophoretic methods in animal tissue homogenates, cell lysates, blood, serum, plasma and bronchoalveolar fluids. Research Infrastructure of the Laboratory Spectrophotometer, ELISA reader, Refrigerated centrifuge, Homogenizer, Horizontal and vertical electrophoresis systems, Transilluminator, Ultrapure water system, Deep freezer -86°C, Vortex. Collaborations and Projects We conduct TÜBİTAK (Scientific and Technological Research Council of Turkey) funded research and development projects on activity mechanisms of therapeutic new molecules in various disease models. Furthermore we have completed national and international patent applications about a new molecule proposed to be used in treatment of some pulmonary diseases. We have collaborations with Department of Chemistry from Faculty of Engineering, Department of Chest Medicine from Cerrahpasa Medical Faculty (Turkey), Marmara University (Turkey), Helmholtz Zentrum München - German Research Center (Germany), University of Antwerp (Belgium), and University of Maryland (USA). Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Füsun Öztay +90 212 4555700 - 15044 fusoztay@istanbul.edu.tr Asst. Prof. Dr. Pelin Arda Pirinççi +90 212 4555700 - 15081 parda@istanbul.edu.tr FACULTY of SCIENCE PRIMARY-STEM CELL and CANCER RESEARCH LABORATORY T his laboratory consists of Primary and Stem Cell Culture Research Laboratory, and Cancer Cell Culture Research Laboratory. In Primary-Stem Cell Culture Research Laboratory, pancreatic beta cells were isolated for the first time in Turkey and single cell studies were conducted by long-term culture applications. Molecular mechanisms affecting beta cells in diabetes are investigated by trying to unveil mechanisms of cell death, damage and repair in diabetes. Our research group, studying primary pancreatic beta cells isolated from in vivo experimental hyperglycemic and diabetic animals and islet-derived mesenchymal stem cells, aim to make a fundamental contribution to therapeutic approaches to human health, and ensure implementation of studies by building a bridge between fundamental sciences and clinical sciences. In addition, mechanisms that may contribute to process of fibrosis in cGRP and GRP-stimulated human pulmonary primary fibroblast cells and mouse primary type II pneumocyte are investigated. In Cancer Cell Culture Research Laboratory, researches on Insulinoma cell line (INS-1), pancreatic ductal cell line ARIP, pancreatic acinar cell line AR42J, breast cancer cell lines (MCF-7 and MDA- MB 231), C6 glioma cell line, follicular, papillary and anaplastic thyroid carcinoma lines are conducted. Research Infrastructure of the Laboratory Laminar flow cabinet, Ultrasonic water bath, Inverted microscope, Autoclave, UV cabinet, Deep freezer -152°C, Flow cytometry. Collaborations and Projects We conduct international collaborative studies with Helmholtz Zentrum München - German Research Center (Germany), Universidad Nacional Autonoma de Mexico (Mexico) and University of Maryland (USA) as well as TÜBİTAK (Scientific and Technological Research Council of Turkey) funded research and development projects. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Selda Gezginci Oktayoğlu +90 21 2 4555700 - 15084 selgez@istanbul.edu.tr Assoc. Prof. Dr. Engin Kaptan +90 212 4555700 - 15089 engkaptan@istanbul.edu.tr 61 FACULTY of SCIENCE RADIOECOLOGY LABORATORY Q 62 ualitative and quantitative analyses of gamma (137Cs, 40K, 232Th and 238U series) and alpha-emit210 ting ( Po) radionuclides in terrestrial and marine samples are performed using gamma spectrometry equipped with a high-purity Ge detector and alpha spectrometry. Sediment core, sediment trap and mussel transplantation methods are used in biomonitoring studies. Furthermore bioaccumulation kinetics of radionuclides and metals in marine invertebrates is studied. Collaborations and Projects We conduct collaborative studies with İstanbul Technical University Faculty of Mines Department of Geology (Turkey) within the scope of TÜBİTAK (Scientific and Technological Research Council of Turkey) project and with Kocaeli University Faculty of Sciences Department of Biology (Turkey) in determination of contaminants (radionuclide, metal and organic) in Golden Horn by active bio-monitoring methods. We work in collaboration with University of La Rochelle (France) and Environmental Laboratory of Monaco (Monaco) on accumulation of radionuclides in marine invertebrates under high CO2 conditions, and with University of Lisbon Institute of Technical Sciences (Portugal) on 226Ra, 238U, 232Th, Pu, 210Po measurements in marine organisms. 238+239 Research Infrastructure of the Laboratory Gamma spectrometer (with a high-purity Ge detector), Alpha spectrometer (with eight ion-implanted silicon alpha detectors with an active area of 300 mm2). Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Murat Belivermiş +90 212 4555700 - 15077 belmurat@istanbul.edu.tr Assoc. Prof. Dr. Önder Kılıç +90 212 4555700 - 15090 okilic@istanbul.edu.tr FACULTY of SCIENCE MOLECULAR CANCER BIOLOGY LABORATORY S tudies on routine cellular passages of normal and tumoral cell lines, and cellular and molecular analyses are conducted. Moreover, cytotoxicity analyses of various antineoplastic agents and plant extracts in cell and tissue culture, cell kinetics parameters (Mitotic Index, Labelling Index and Apoptotic Index), DNA damage and repair pathways, cell cycle and expressions of genes involved in cell death types as well as radiosensitivity and chemosensitivity studies are conducted. Research Infrastructure of the Laboratory Optical microscope (phase-contrast and fluorescence), Laminar flow cabinet, CO2 incubator, Refrigerated centrifuge, PCR system, Electrophoresis system, Qiaxcel imaging device (As many as 96 samples can be analyzed at as low concentrations as 3-5 base pair). Collaborations and Projects We conduct joint studies with American Association for Advancement of Science (USA), European Association for Cancer Research (UK) and Migal-Galilee Research Center (Israel). Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Gül Özcan Arıcan +90 212 455 57 00 - 15093 gozcan@istanbul.edu.tr 63 FACULTY OF SCIENCE IMMUNOHISTOCHEMISTRY RESEARCH LABORATORY V arious studies are conducted using TUNEL method where apoptotic cells are exhibited by immunohistochemical labeling of enzymes and hormones in appropriate tissues in different experimental models, including primarily diabetes, sepsis, epilepsy and cancer induced in mice and rats. Research Infrastructure of the Laboratory Rotary microtome, Microscopic image analysis system. Collaborations and Projects We work in collaboration with University of Amsterdam Academic Medical Center (The Netherlands), Bezmialem Foundation University (Turkey) and Giresun University (Turkey). We also conduct projects where immunohistochemical methods are 64 used in various tissues sampled from different experimental models as part of TÜBİTAK (Scientific and Technological Research Council of Turkey) projects. Laboratory Supervisor and Contact Information Prof. Dr. Kadriye Akgün Dar Prof. Dr. Cihan Demirci Tansel +90 212 4555700 – 15101 +90 212 4555700 – 15099 cihan@istanbul.edu.tr kakgun@istanbul.edu.tr FACULTY of SCIENCE PHYSIOLOGY RESEARCH LABORATORY W e perform physiological studies, circulatory and nervous system applications, microcirculation studies, investigation of effects of various endogenous and exogenous agents against acute and/or chronic ischemia/reperfusion injury and studies on determination of cardiac parameters in Langendorff Isolated Heart System, and various studies regarding neurodegenerative diseases on different animal models (sepsis, diabetes, hypertension, hyperglycemia, epilepsy, hemorrhagic shock etc.) by real time measurements under anesthesia. Research Infrastructure of the Laboratory Langendorff isolated heart system, Homogenizer, ultra-pure water purification system, Powerlab 8Sp chart recorder and scope software, Bio amplifier, bridge amplifier, Blood pressure transducer, Blood flowmeter, Fine needle probe, Infusion pump, Ventilator, Stereotactic surgery system. Collaborations and Projects We conduct collaborative studies with University of Amsterdam Academic Medical Center (The Netherlands), Bezmialem Foundation University (Turkey), Giresun University (Turkey) and Karadeniz Technical University (Turkey). We also conduct projects on effects of various agents in Langerdorff Isolated Heart System and septic shock in vivo electrophysiological data acquisition system and circulatory and renal functions in different experimental models as part of TÜBİTAK (Scientific and Technological Research Council of Turkey) projects. Laboratory Supervisor and Contact Information Prof. Dr. Cihan Demirci Tansel +90 212 4555700 – 15099 cihan@istanbul.edu.tr 65 FACULTY of SCIENCE MOLECULAR RADIOBIOLOGY and BIOMOLECULAR TECHNOLOGY RESEARCH LABORATORY I 66 n preclinical cancer research, besides treatment of cancer by chemotherapeutic agents and various extracts, regulation of treatment of cancer by cellular aging, aromatase inhibitors, nanotechnological drugs and hyperthermia are investigated as well. Studies on subjects such as Labelling Index, Mitotic Index, Apoptotic Index, and Cell Index, Mitochondrial dehydrogenase activity assay, tyrosine kinase activity assay, hyperthermia applications and evaluation continue. Research Infrastructure of the Laboratory Laminar flow cabinet, Inverted microscope, Optical microscope, Centrifuge, Deep freezer -20oC, Deep freezer -70oC, Precision scales, Distilled water system, xCELLigence Real Time Cell analysis system, CO2 incubator, Shaker incubator, MagCellect devices. Collaborations and Projects We conduct joint studies with Marmara University Faculty of Pharmacy (Turkey), İstanbul Faculty of Medicine (Turkey), İstanbul University Cerrahpasa Faculty of Medicine (Turkey), İstanbul University Faculty of Pharmacy (Turkey) and American Association for the Advancement of Science (AAAS), American Society Therapeutcal Radiology and Oncology (ASTRO) and European Society Therapeutical Radiology and Oncology (ESTRO). Laboratory Supervisor and Contact Information Asst. Prof. Dr. Mehmet R. Topçul +90 212 4555700 - 15097 topcul@istanbul.edu.tr FACULTY of SCIENCE PLANT BIOTECHNOLOGY and GENETICALLY MODIFIED ORGANISMS LABORATORY Q ualitative and quantitative analysis of genetically modified organisms (GMO), reproduction of certain gene regions, quantitative determination of functional genes, determination of gene expression and analysis of specific genes are performed using RT-PCR. In addition, studies such as DNA, RNA, protein isolations, Southern, Northern and Western blotting, gel electrophoreses and imaging of protein and nucleic acids, microproduction of endemic or endangered plants by tissue culture, and determination of polymorphism and phylogenetic relationships between species by molecular marker techniques are conducted. Collaborations and Projects TÜBİTAK (Scientific and Technological Research Council of Turkey) funded projects and COST projects as well as other EU-supported collaborative studies on molecular approaches for increasing starch synthesis of plants which is of nutritional, industrial and economic importance with Koç University (Turkey), “Taxonomic Revision of Campanula: Sections Symphyandriformes, Rupestres, Scapiflorae, Saxicolae, Tracheliopsis, Dictyocalyx and Platysperma and their Phylogenetic relationships in Turkey and 2012COST ACTION: Omics Technologies for Crop Improvement, Traceability, Determination of Authenticity, Adulteration and Origin in Saffron continues. This laboratory has a potential to conduct routine GMO analyses. We conduct collaborative studies with National Institute for Scientific Agricultural Research (France) for development of new GMO Research Infrastructure of the Laboratory analysis methods. Real-time PCR, Nanodrop 2000 spectrophotometer, Geliance 200 imaging system, 3 - 30 K ultracentrifuge. Laboratory Supervisor and Contact Information Prof. Dr. Gül Cevahir Öz Prof. Dr. Mahmut Çalışkan +90 212 4555700 – 26808 cevahirg@istanbul.edu.tr +90 212 4555700 - 26850 mahmut.caliskan@istanbul.edu.tr 67 FACULTY of SCIENCE ENVIRONMENTAL and INSTRUMENTAL ANALYSIS LABORATORY D 68 etermination of elemental contents of plants, soil, water and sediment samples, purifications, characterization of peptides and proteins, fractionation of such molecules as amino acids, vitamins, such contaminants as pesticides and polycyclic aromatic compounds have been conducted and detection studies for such various chemical compounds as volatile oils and fatty acids in samples and high molecular weight biopolymers are performed. Research Infrastructure of the Laboratory ICP-optical emission spectrometer, Ultrahigh performance liquid chromatography, Gas chromatography-mass spectrometry, Microwave oven system, Fat extractor. Collaborations and Projects We conduct collaborative studies with Yıldız Technical University (Turkey), University of Orleans (France), Bielefeld University (Germany), University of Oslo (Norway) and University of Debrecen (Hungary). TÜBİTAK (Scientific and Technological Research Council of Turkey) funded projects and research and development projects within the scope of University-Industry Cooperation are being conducted and the laboratory has a potential and capacity to perform routine analyses. Laboratory Supervisor and Contact Information Prof. Dr. Gül Cevahir Öz +90 212 4555700 – 26808 cevahirg@istanbul.edu.tr Assoc. Prof. Dr. Gülriz Bayçu Kahyaoğlu +90 212 4555700 – 26808 gulrizb@istanbul.edu.tr FACULTY of SCIENCE FUNDAMENTAL and INDUSTRIAL MICROBIOLOGY RESEARCH LABORATORY W e conduct studies on isolation and identification of Legionella, sulphate-reducing bacteria and sulphur-oxidating bacteria, free living amoeba, microbiological corrosion, determination of antibacterial activity, disinfectant effectiveness studies (suspension, biofilm), microbiological food analysis, isolation of bacteria reducing sulphur from petroleum and methane-producing bacteria, biological quality determination of various products, mycology, aerobiology, determination of internal and external air quality, water pollution, quorum sensing, biofilm tests and characterization in man-made water systems. Research Infrastructure of the Laboratory Identification of bacteria (Epifluorescence microscope, Gradient PCR), Community analysis (Denatured gradient gel electrophoresis), Anaerobic culture methods (Glovebox), Electrochemical microbiological corrosion trials (Potentiostat-Galvanostat device), Environmental molecular microbiology studies (Nanodrop-ELISA reader), Microbiological air quality (Air sampler). Collaborations and Projects We have academic collaborations with Yıldız Technical University (Turkey), Boğaziçi University (Turkey), Trakya University (Turkey), Delft University of Technology (The Netherlands), Flinders University (Australia), and Turkish Petroleum Corporation (TPAO). We conduct projects on microbiological characterization of petroleum produced in Turkey, microbiological corrosion of metals in cooling towers within the scope of project funded by TÜBİTAK (Scientific and Technological Research Council of Turkey) and make preparations for commercialization and patent applications for anti-biofilm nanotechnological coatings. Laboratory Supervisor and Contact Information Prof. Dr. Ayşın Çotuk +90 212 4555700-15069 abcotuk@istanbul.edu.tr 69 FACULTY of SCIENCE MOLECULAR RADIOBIOLOGY and PLANT BIOTECHNOLOGY RESEARCH LABORATORY P 70 utative-mutant plants, with tolerance to various abiotic stresses, including drought, salinity and herbicides have been produced in various plants, including sugar beet, potato, wheat and soybean by using a combination of Plant Mutation Breeding and Molecular Biology techniques. Gamma irradiation of in vitro and in vivo samples has been used to improve these target traits. Desired mutants are screened using molecular and biochemical techniques. Various plant species, which have agricultural and economic importance, are propagated by plant tissue culture techniques. Research Infrastructure of the Laboratory Laminar flow cabinet, Incubator, Precision scales, PCR cycler, Nanophotometer, Growth cabinet (Nuve), Plant growth cabinet (Sanyo), Centrifuge, Gel running vertical and horizontal electrophoresis tanks. Collaborations and Projects We have an ongoing collaboration with Join FAO/ IAEA Nuclear Techniques in Food and Agriculture, Plant Breeding and Genetics Laboratory (Austria). Laboratory Supervisor and Contact Information Asst. Prof. Dr. Sema Alikamanoğlu +90 212 455 57 00 - 15094 semakaman@istanbul.edu.tr FACULTY of SCIENCE PLANKTON CULTURE LABORATORY W e conduct studies on diagnosis and production of dinoflagellate cysts found particularly in sea sediment within the frame of production and culturing of microscopic organisms found in our waters in laboratory environment. Research Infrastructure of the Laboratory Incubator, autoclave, Ultrasonic bath, Precision scales, Photometer, Optical microscope and imaging system. Collaborations and Projects International bilateral collaboration project “Microalgae resting cysts in recent marine sediments and their role in establishment of Harmful Algal Blooms (HABs) in three eutrophicated bays in Greece and Turkey” supported by TUBITAK (Scientific and Technological Research Council of Turkey) -GSRT (Greece) partnership has been conducted. Laboratory Supervisor and Contact Information Prof. Dr. Neslihan Balkıs Asst. Prof. Dr. Benin Toklu Alıçlı +90 212 455 57 00 - 15115 neslbalk@istanbul.edu.tr +90 212 455 57 00 - 15147 benin@istanbul.edu.tr 71 FACULTY of SCIENCE ISTF (HERBARIUM) I t is an international herbarium registered in Index Herbariorum since 1956. Every year, national and international researchers visit herbarium to conduct studies on more than 40000 plants including samples of a high historical and scientific value. Research Infrastructure of the Laboratory Scanning Electron Microscope (Jeol JCM 5000 Neoscope Benchtop), Photomicroscope and Stereomicroscope (Olympus SZX16)/Photography and measurement, Optical microscopes and stereomicroscopes for dissection Collaborations and Projects It is in contact with all formal and registered herbariums in the world and admits visitors. 72 Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Osman Erol +90 212 4555700 - 26820 erol@istanbul.edu.tr FACULTY of SCIENCE NANO and OPTOELECTRONIC RESEARCH LABORATORY O ptical and electrical characterization of novel semiconductor materials and electronic/optoelectronic devices based on semiconductors at a wide range of temperature (2 – 300 K), investigation of charge transport phenomena in solid materials by magneto-transport, detection of defect levels, synthesis and characterization of nanostructures and investigation of magnetic properties of semiconductors are the main research activities. Collaborations and Projects We conduct collaborative studies with Anadolu University (Turkey), Cumhuriyet University (Turkey), Sakarya University (Turkey), Akdeniz University (Turkey), University of Essex (UK), Tampere University of Technology (Finland), LAAS (France), and Victoria University (Canada). We conduct TÜBİTAK (Scientific and Technological Research Council of Turkey) funded projects on electrical/optical properties of novel semiconductor alloys, design and characterization of photodetectors for optical Research Infrastructure of the Laboratory Optical characterization (spectral photoconductivity, spectral photovoltage, reflection, modulation spec- communication and COST projects on development of novel gain materials/devices based on semiconductors alloys and devices operating in THz region. troscopy, photoluminescence) from UV (200 nm) to IR (10 µm) region between 2 and 300 K, Electrical characterization (a.c. and d.c. Current-Voltage Measurements), Hall Effect measurement system (2.3 T, 10 - 300 K), Magneto-transport set-up (7 T, 4 - 300 K), Defect spectroscopy (PITS), Liquid nitrogen generator with a production capacity of 40 l/day. Laboratory Supervisor and Contact Information Prof. Dr. Ayşe Erol +90 212 4555700 - 15005 ayseerol@istanbul.edu.tr 73 FACULTY of SCIENCE ADVANCED LITHOGRAPHIC METHODS LABORATORY A 74 dvanced Lithographic Methods Laboratory has been established in 2014 as an outcome of Ministry of Development-funded infrastructure project and comprises of a clean room area of 100 m2 with Class 10, 100 and 1000 sections. The laboratory facilities are suitably equipped to enable studies on fabrication of electronic/ optoelectronic devices by photolithography and dry etching techniques at a nano/micro scale, imaging of surfaces and cross-sections of solids and determination of elemental composition of materials. Research Infrastructure of the Laboratory Wet bench equipped with spin coater, Hot plate, Ultrasonic cleaner, Chemical bench, Mask aligner (SUSS MJB4), Thermal evaporator (VAKSIS), Profilometer (KLA Tencor P6), Wire bonder (Kulicke &Soffa), Dry etching equipment (FEI Versa 3D Dual Beam Focused Ion Beam / RAITH Electron beam system), SEM (FEI), AFM (Park Systems), EDS (FEI). Collaborations and Projects It is aimed to initiate and conduct collaborative research with Anadolu University (Turkey), Cumhuriyet University (Turkey), Sakarya University (Turkey), Akdeniz University (Turkey), University of Essex University (UK), Tampere University of Technology (Finland), LAAS (France) and Victoria University (Canada) on fabrication and characterization of novel semiconductor structures and optoelectronic/ electronic devices. Laboratory Supervisor and Contact Information Prof. Dr. Ayşe Erol +90 212 4555700 - 15005 ayseerol@istanbul.edu.tr FACULTY of SCIENCE HIGH MAGNETIC FIELD and LOW TEMPERATURE LABORATORY H igh Magnetic Field and Low Temperature Laboratory, founded in 2012 as an outcome of a Istanbul University-funded project, is suitably equipped to enable studies on investigation of charge transport mechanisms in solid materials and semiconductor devices at a variable magnetic field and low temperatures, magnetic field-dependent conductivity, classical Hall Effect and quantum Hall Effect, electrical characterization and investigation of magnetic properties of solids. Research Infrastructure of the Laboratory 18/20 T Superconducting magnet system with a dilution refrigerator (10 mK), Superconducting magnet system (7 T, 4.2 - 300 K), Helium recycling system and helium liquefier system (18 l/day). Collaborations and Projects It is proposed to conduct collaborative studies and projects with University of Essex (UK), Tampere University of Technology (Finland), LAAS (France), INSA (France) and Victoria University (Canada) based on investigation of magneto-transport properties of semiconductor materials, classical and quantum Hall Effects, charge transport in solids. Laboratory Supervisor and Contact Information Prof. Dr. Ayşe Erol +90 212 4555700 - 15005 ayseerol@istanbul.edu.tr Asst. Prof. Dr. Saffettin Yıldırım +90 212 4555700 – 15424 safyield@istanbul.edu.tr 75 FACULTY of SCIENCE MOLECULAR SPECTROSCOPY LABORATORY C 76 haracterization of molecular structures, identification of functional groups and determination of crystallinity are among the main activities of this laboratory. The structural analysis of the drugs used in the treatment of important diseases and their interactions with the media are being studied spectroscopically. Detection of the effects of various diseases at molecular level is also aimed (identification of cancerous tissues, studies on osteoporosis etc.). In addition, the structural analysis of archaeological findings and determination of the chemical compositions of semiconductor alloys are included in its range of activities. All of these experimental studies are further supported by molecular simulation calculations. Research Infrastructure of the Laboratory Fourier Transform Infrared Spectrometer (Medium and near-IR), Fourier Transform Raman Spectrometer (50 – 3600 cm-1 spectral range, 1064 nm wavelength and 1 W laser source), Micro Raman dispersive spectrometer (50 – 8000 cm-1 spectral range, 785 nm and 532 nm wavelength and 100 mW laser sources). Collaborations and Projects We have collaborative studies with University of Potsdam (Germany), Oral Roberts University (USA), Uzhgorod University (Ukraine) and Politechnico di Milano (Italy). Molecular structure and vibrational analysis studies as part of TÜBİTAK (Scientific and Technological Research Council of Turkey) projects, and structural analysis and electronic band gap studies of amorphous solids as part of a TÜBİTAK International Bilateral Cooperation project are among he conducted projects. Laboratory Supervisor and Contact Information Prof. Dr. Ayşen E. Özel Prof. Dr. Elif Akalın +90 212 4555826 aozel@istanbul.edu.tr +90 212 4555700 - 15445 eakalin@istanbul.edu.tr FACULTY of SCIENCE LASER SPECTROSCOPY LABORATORY L aser Spectroscopy Laboratory, established in 2012 with the support of Istanbul University, currently deals with determination of hyperfine structure constants from atomic spectra of elements, and determination of unknown spectral lines of atoms. Hyperfine structure constants are also used in astrophysics. Accordingly, it is an area befitting interdisciplinary studies. Research Infrastructure of the Laboratory In order to examine the hyperfine structure of atoms and to find new energy levels, Laser Induced Fluorescence Spectroscopy (LIF), Saturated Absorption Spectroscopy (SAS) and Opto-galvanic Spectroscopy (OGS) methods are applied by using a tunable cw Titan Sapphire laser pumped by an 18 W optical power, 532 nm wavelength, Cw solid-state laser (having an optical power of 4 W operating within 700 and 1100 nm range, Continuously tunable at 30 GHz). 77 Collaborations and Projects We have bilateral collaborative projects and studies with HTW Berlin (Germany), Latvia University (Latvia), Graz Technical University (Austria) and Poznan Technology University (Poland), aimed at experimental and theoretical identification of fine structure parameters and hyperfine structure constants of atoms, and determination of new energies of atoms. Laboratory Supervisor and Contact Information Prof. Dr. Gönül Başar +90 212 4555843 gbasar@istanbul.edu.tr FACULTY of SCIENCE DIELECTRIC SPECTROSCOPY LABORATORY D 78 ielectric Spectroscopy Laboratory, established in 2012 with the support of Istanbul University, is capable of producing oxide and polymer materials by using different techniques such as electron beam, thermal evaporation, rf and magnetron sputtering. Dielectric characteristics (capacity, dielectric loss, AC conductivity, relaxation time, etc.) of bulk and powder materials, thin films, polymers and liquid samples within 3 µHz – 20 MHz frequency and 173 K – 373 K temperature ranges, and DC conductivity of bulk and thin films are studied. Research Infrastructure of the Laboratory A vacuum system (10-6 Torr with a thickness monitor) and Dielectric Conductivity and Impedance Measurement System operating within 3 µHz – 20 MHz frequency and 173 K – 373 K temperature ranges and ELGA Purelab Options-S7/15 water purification system. Collaborations and Projects We have collaborative projects and studies with Marmara University (Turkey) and Bahçeşehir University (Turkey) on growth and characterization of materials for industrial needs, biological liquids, and an international collaboration with Charles University (Czech Republic). Laboratory Supervisor and Contact Information Prof. Dr. Deniz Değer Ulutaş +90 212 4555700 - 15292 deger@istanbul.edu.tr FACULTY of SCIENCE NUCLEAR PHYSICS RESEARCH LABORATORY N uclear Physics Research Laboratory was established in 2014 with the support of Istanbul University. Nuclear Physics Laboratory consists of Gamma Spectroscopy and Radon Measurement Laboratories. Gamma Spectroscopy Laboratory can handle vast variety of samples including food (tea, hazelnuts, etc.), drink, industrial materials and products (fertilizers, coal, cement, ceramic, marble, granite, etc.) for preparation and measurements. Radon Measurement Laboratory is capable of performing active and passive radon concentration measurements in soil, water and air samples and carries out research activities related with environmental radiation safety, earthquake – radon relation. Research Infrastructure of the Laboratory A high resolution Gamma Spectroscopy System composed of a p-type Co-axial Ge (HPGe) detector with 35% efficiency for gamma energies between 40 keV to 10 MeV, Solid state nuclear alpha track detectors for passive radon measurements and an AlphaGuard for active radon concentration measurements in soil, water and air. Collaborations and Projects This laboratory is suitably equipped for future collaborations. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Latife Şahin Yalçın +90 212 4555700 - 15410 latife.sahin@istanbul.edu.tr 79 FACULTY of SCIENCE ANCIENT DNA RESEARCH LABORATORY D NA isolations from fossils (human, animal, plant, etc.) found in archeological sites, and at later stages, sex-determination and analyses of mitochondrial, X and Y chromosome series are carried out. The resulting series information is bioinformatically assessed, and Haplo-group studies are conducted. Research Infrastructure of the Laboratory Gradient PCR and classical PCR systems, Computers and bioinformatics softwares. Collaborations and Projects We have collaborative studies and projects with Trakya University (Turkey), Süleyman Demirel University (Turkey), International Society for Computational Biology (Italy) and International Center for Genetic Engineering and Biotechnology (Italy), and ongoing 80 projects with the Ministry of Science, Industry and Technology. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Ercan Arıcan +90 212 4555700 - 15119 earican@istanbul.edu.tr FACULTY of SCIENCE PLANT BIOTECHNOLOGY LABORATORY C ultivation studies are being conducted on barley (Hordeum vulgare L.), bread wheat (Triticum aestivum L.), rice (Oryza sativa L.), tobacco (Nicotiana tabaccum L.) and sunflower (Avena sativa L.), as model organisms for plants. Somatic variations are studied on barley under tissue culture conditions and especially at transposon level, and gene expression analyses and transposon analyses are conducted on transgenic tobacco plants, and phenological, physiological and genomic responses are analyzed and correction of these responses by plant hormones is studied on barley, wheat and sunflower under abiotic stress circumstances (salinity and heavy metals, etc.), and genetic and epigenetic methods are used therein for. GMO analyses are carried out on rice plant. Our Laboratory further uses its expertise on molecular markers in genetic and epigenetic analyses of geriatric genes in human. Research Infrastructure of the Laboratory Plant growth cabinets, Horizontal electrophoresis systems, PCR systems (classical and real-time), Autoclaves, Optical microscopes, Fluorescent microscope, Confocal microscope. Collaborations and Projects We have collaborative studies and projects with Institute for Biotechnology and Genetic Engineering (Bosnia-Herzegovina), Sofia University (Bulgaria) and Marmara University (Turkey). Laboratory Supervisor and Contact Information Prof. Dr. Nermin Gözükırmızı +90 212 4555700 - 15111 nermin@istanbul.edu.tr 81 FACULTY of SCIENCE PROTEOMICS LABORATORY P 82 roteomic analyses (1D, 2D Electrophoresis and MS analyses) are conducted on various biological materials (plants, yeasts, animal cells, stem cells, etc.) and on clinical samples. Bioactivities of proteins and peptides (especially HSPs, allergenic pollen proteins, milk proteins and hydrolysis products) are determined, and electrophoretic / chromatographic separation techniques and Western-blot analysis are carried out. Activities of various enzymes such as SOD, catalase, peroxidase, IMPDH and caspases are measured. In recombinant cells and clinical samples developed by genetic engineering techniques, expression of oxidative stress and apoptosis-related genes is examined, and their protein profiles are compared. Research Infrastructure of the Laboratory Vertical and horizontal electrophoresis systems, HPLC, Isoelectric focusing device, Micro-rotator fractionation device, Gel imaging systems, Gel drying system, Automated electrophoresis system (Experion), Protein purification system (Profinia), Spectrofluorometer, Quibit Spectrofluorometer, Refrigerated centrifuge, Transblotter, Western-Blot System, Digital PCR system, Fluorescent microscope, Freeze dryer. Collaborations and Projects We have international collaborative studies and projects with Edinburgh University (UK), Joseph Fourier University and Promethee Proteomic Platform (France), Cologne University (Germany), Marmara University (Turkey), Ege University (Turkey), Adıyaman University (Turkey), Kocaeli University KABI Proteomics Laboratory (Turkey), TÜBİTAK (Scientific and Technological Research Council of Turkey) – Marmara Research Center Genetic Engineering and Biotechnology Institute. Laboratory Supervisor and Contact Information Prof. Dr. Nazlı Arda +90 212 4555700 - 15113 narda@istanbul.edu.tr Asst. Prof. Dr. Evren Önay Uçar +90 212 4555700 - 15117 evrenonay@istanbul.edu.tr FACULTY of SCIENCE MOLECULAR DIAGNOSIS in HUMAN DISEASES LABORATORY O ur laboratory is engaged in intensive research on prenatal diagnosis by fetal genomic analysis methods, and is trying to clarify fetal physiology. Genetic diseases that can be diagnosed by non-invasive prenatal diagnosis methods with microarray technology are researched, in addition to molecular genetic analyses for diagnosis and treatment of genetic origin diseases. Studies aimed at finding a new molecular marker that can be used for diagnosis of pregnancy complications such as pre-eclampsia are ongoing. Blood group of fetus is determined through amniotic fluid, and existence of RHD gene (blood incompatibility) of baby can be detected in maternal blood. In cooperation with specialized doctors, gonadic and sex-linked diseases can be diagnosed respectively from amniotic fluid and maternal blood. Furthermore, through analysis of polymorphisms and sports-susceptibility genes in genome, we are trying to make contri- bution to sports and exercising. Research Infrastructure of the Laboratory Real-time PCR systems, Microcentrifuge, Hybridization oven, Pikofuge, Microarray scanner, Bioanalyzer, DNA/RNA extraction device. Collaborations and Projects We have international collaborative studies and projects with Sweden Genome Center (Sweden), in addition to TÜBİTAK -supported projects, and collaborations with some private clinics and SMEs. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Tuba Günel +90 212 4555700 - 15473 gunel@istanbul.edu.tr 83 FACULTY of SCIENCE PLANT GENOMIC RESEARCH LABORATORY T 84 his laboratory conducts studies on Hordeum spontaneum and Brachypodium types of barley (Hordeum vulgare L.), a model organism for plants. Phenological, physiological and genomic responses of plants under stress conditions are studied by molecular methods. Expressions and allelic variations of genes of different pathways under conditions of drought, salinity and combined stress are examined. The studies aimed at increasing transformation frequency in monocot cells through Agrobacterium tumefaciens-mediated gene transfer are also carried out. Polymorphism studies based on IGS-RFLP and SSR markers are conducted on our country-origin pathogen and endophyte Fusarium types, and distribution, pathogenicity levels and use of endophytes in agriculture are researched. Changes caused by Fusarium infection in barley roots are examined by physiological and genomic tools. Research Infrastructure of the Laboratory Plant growth cabinets, Electroporator system, Osmometer, Conductivity meter, Incubator, Microscope. Collaborations and Projects We have COST projects and collaborative studies with Perugia University (Italy), Ondokuz Mayıs University (Turkey), and Ebittek Biotechnology R&D Ltd. company. Laboratory Supervisor and Contact Information Prof. Dr. Filiz Gürel +90 212 4555700 - 15472 filiz@istanbul.edu.tr FACULTY of SCIENCE BIOINFORMATICS LABORATORY B ioinformatics laboratory carries out research on various different DNA structures (Z-DNA, G-Quadruplex, etc.) in Yeast (S. cerevisiae and S. pombe) genome, and determines interactions between the relevant structures and gene functions, and compares mitochondrial genome X and Y chromosome series, and in the light of the resulting information, assesses phylogenetic information. The studies on identification of bioinformatics-supported molecular markers in plants are also carried out. Research Infrastructure of the Laboratory Gradient PCR and classical PCR systems, Computers and bioinformatics softwares. Collaborations and Projects We have collaborative studies and projects with International Society for Computational Biology (Italy) and International Center for Genetic Engineering and Biotechnology (Italy), and some ongoing projects with the Ministry of Science, Industry and Technology. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Ercan Arıcan +90 212 4555700 - 15119 earican@istanbul.edu.tr 85 FACULTY of SCIENCE GENETICALLY MODIFIED ORGANISMS LABORATORY M 86 olecular studies are conducted on foreign genes in microorganisms, in addition to plants among the genetically modified organisms. For the purpose of GMO diagnosis in plant systems, DNA isolation from processed and unprocessed food stuff, and foreign gene screening by qualitative and quantitative PCR, and quantification of foreign genes, and typology of foreign genes continue. Furthermore, studies are carried out for genomic level determination of manipulations, which are aimed at the change of gene expression in plants and plant products. As part of biosafety, transformation frequency determination studies are carried out in microbial system models for horizontal gene passage from plant organisms to animals and to soil microorganisms. Research Infrastructure of the Laboratory PCR system, Vertical and horizontal gel electrophoresis systems. Collaborations and Projects We have collaborative studies and projects with European Union Joint Research Center (Italy), Biosafety Board (Turkey), Union of Seed Raisers (Turkey), Crop Central Research Institute (Turkey), in addition to TÜBİTAK (Scientific and Technological Research Council of Turkey) supported ongoing projects. Laboratory Supervisor and Contact Information Prof. Dr. Şule Arı Dr. Özgür Çakır +90 212 4555700 - 15108 sari@istanbul.edu.tr +90 212 4555700 - 15139 ozgurckr@istanbul.edu.tr FACULTY of SCIENCE PLANT PATHOLOGY LABORATORY R esearch is conducted on more than 70 isolates of 4 types of Fusarium fungi leading to Fusarium culmorum (WG Smith) Sacc. and Pc / Phyphtora capsici in barley, wheat and corn plants of different geographical regions. Also, ongoing are genotyping of (chromosomal and mitochondrial) isolates by using RAPD, CAPS, PCR-RFLP, SCAR, ISSR and SSR molecular marker techniques. As part of chemotyping of fungi, mycotoxin generation potentials of fungi are analyzed on genomic (PCR-based) and transcryptomic (RT-PCR and real time-PCR) levels, and existence of generated mycotoxins is identified by thin layer chromatography analysis. As a part of gene-silencing studies on Fusarium types, synthetic siRNA molecules are designed specifically for genes related to toxin generation, and furthermore, silencing vectors carrying the copies of these genes are developed. Genetic manipulations in Fusarium types are carried out at co-transformation level, and transformants are examined at morphological, genomic, transcryptomic, metabolomic and spectrofluorimetric levels. Research Infrastructure of the Laboratory Laminar flow cabinet, Temperature and light controlled plant growth cabinet, Shaker incubator, PCR system, Electroporation system, Optical microscope. 87 Collaborations and Projects We have an ongoing collaboration with Isfahan University of Technology (Iran). Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Gülruh Albayrak +90 212 4555700 - 15134 gulruh@istanbul.edu.tr FACULTY of SCIENCE PLANT SECONDARY METABOLISM ENGINEERING LABORATORY F 88 or overproduction of plant secondary metabolites characterized as biological active drug substances, callus, cell suspension and hairy root cultivations and genetic transformation studies (Agrobacterium mediated and electroporation) are conducted. For production of phenolic substances at pilot level, actions are taken for optimization of physicochemical cultivation conditions and for biotechnological production by elicitation. For identification of antioxidant and cytotoxic, etc. activities of plant secondary metabolites, not only enzyme activity tests and molecular analyses, but also analyses for secondary metabolite identification are carried out together with project partners. Furthermore, metabolism of selenium where anti-cancer ingredients are generated is also examined at physiological, genetic (identification and cloning of genes operating at selenium metabolic pathway), genomic and bioinformatics levels. Research Infrastructure of the Laboratory PCR system, Ultra-purified water device, Nanodrop spectrophotometer, Shaker incubator. Collaborations and Projects Projects supported by East Carolina University (USA), TÜBİTAK (Scientific and Technological Research Council of Turkey) and the Ministry of Science, Industry and Technology are being conducted. We have collaborative studies and projects with Ege University (Turkey), Koç University (Turkey), Sabancı University (Turkey) and Istanbul University Teknokent NGS Biotechnology and Sativa Biotechnology companies (Turkey). Laboratory Supervisor and Contact Information Prof. Dr. Şule Arı +90 212 4555700 - 15108 sari@istanbul.edu.tr Asst. Prof. Dr. Neslihan T. Kara +90 212 4555700 - 15139 neslihantk@istanbul.edu.tr FACULTY of SCIENCE YEAST GENETICS, TECHNOLOGY and GENOMIC RESEARCH LABORATORY I t is inquired how eucaryotic cells perceive their environment, and how they regulate their biological processes in response thereto. Accordingly, such metabolic pathways as glucose sensing, transportation into cell, signal transmission, stress response pathways, synthesis and destruction of depot carbohydrates, and thiamine and purine metabolisms are researched and studied at gene, genome and biochemical levels by a combination of classical yeast genetic methods and molecular techniques. In addition, studies are conducted for optimization of enzymes to be used in textile industry for production by fermentation and chronological aging processes at cellular metabolism levels. ies for determination of functions of animal and plant origin genes. Research Infrastructure of the Laboratory Eukaryotic cell counter, Real Time and classical PCR systems, Fermenter, Cell fractionation tools, Gel imaging systems, UV sterilization cabinet. Collaborations and Projects We have collaborative studies and projects with International Society for Computational Biology (Italy) and International Genetic Engineering and Biotechnology Center (Italy), and ongoing TÜBİTAK (Scientific and Technological Research Council of Turkey) supported projects and projects with SMEs. Studies are ongoing for creation of a S. pombe deletion racial library in terms of various different genes. Furthermore, they are also used as host in clarification of various metabolic pathways and in heterologous gene expression studLaboratory Supervisor and Contact Information Assoc. Prof. Dr. Bedia Palabıyık Asst. Prof. Dr. Semian Karaer Uzuner +90 212 4555700 - 15467 bediag@istanbul.edu.tr +90 212 4555700 -15108 semka@istanbul.edu.tr 89 FACULTY of ENGINEERING WASTE TECHNOLOGY RESEARCH LABORATORY S 90 tudies are conducted for safe and stable storage of mine tailings above ground and underground. Included among the analyses and studies carried out in our laboratory are particle size distribution analysis of waste materials, chemical element content analysis, determination of particle shape, compaction curves and optimum intensity analysis, liquid limit analysis, porosity, permeability, slump and water contents, sedimentation and filtration, pipe flow tests, channel flow tests, yield stress, compressive strength, shearing stress, process water quality analysis, viscosity, liquefaction, consolidation, desiccation cracks intensity analysis, and water holding (retention) curves analysis. At the same time, for simulation of behavior of pasty materials at storage site, laboratory measurements and analyses are effected under field conditions simulated in laboratory waste cabinets. Research Infrastructure of the Laboratory Tension/compression resistance testing machine, Ground compactor device, Ground sampling set, Atterberg consistency limits experiment set, Falling-head permeability experiment set, Shear box test device, Consolidation experiment set, CBR test device, Waste cabinets of various different sizes, Temperature – volumetric water content sensors, Water absorption potential sensors, oxygen sensors, Elemental analysis (XRF) device, Rheometer device, Cure cabinet, Laboratory-type mixer, Slump experiment set. Collaborations and Projects We have collaborative studies and projects with Quebec University (Canada). Laboratory Supervisor and Contact Information Prof. Dr. Ataç Başçetin +90 212 4737070 - 19409 atac@istanbul.edu.tr FACULTY of ENGINEERING ORGANIC CHEMISTRY RESEARCH LABORATORY R eactions of polyhalogenic compounds and haloquinones with amines, alcohols and thiols in various different solvents (protic, aprotic, etc.) in presence of acid or base are examined, and new organic compounds are synthesized, and chromatographic chemical and physical characteristics of synthesized compounds and complexes are determined, and structural clarification studies are conducted. Research Infrastructure of the Laboratory FTIR (400 – 4000 cm-1), Potentiostat / galvanostat / ZRA System (60 pA – 600 mA), UV-Vis spectrometer (200 – 900 nm), Fluorescence spectrophotometer (200 – 900 nm), Polarimeter, Horizontal or vertical condenser rotating evaporation systems, Microdistillation unit, Platform purification device. Collaborations and Projects We have completed a project for synthesis, characterization and activity qualification of new biologically active substances together with Lviv Polytechnical University (Ukraine) as a part of an internationally funded TÜBİTAK (Scientific and Technological Research Council of Turkey) project. Furthermore, Ministry of Development projects have also been completed in connection therewith. Laboratory Supervisor and Contact Information Prof. Dr. F. Serpil Göksel +90 212 4737070 – 17779 segoksel@istanbul.edu.tr 91 FACULTY of ENGINEERING FOOD ANTIOXIDANTS MEASUREMENT APPLICATION and RESEARCH CENTER R 92 eporting of total antioxidant capacity values of food stuffs (all kinds of solid and liquid foods) produced and processed in our country, and labeling according to the Turkish Food Codex Regulation, and scavenging activity assay of food antioxidants towards reactive oxygen types are among the activities of our laboratory. Antioxidant contents of food stuffs are reported and labeled by means of CUPRAC antioxidant capacity / activity assay method. Through identification and separation of antioxidant-rich fractions of foods, new diet and nutrition suggestions abundantly containing these compounds are formulated. trospray [ESI] ion source with a mass measurement range of 2-2000 a.m.u. and a scanning rate of 10000 a.m.u./sec and an ion source capable of Multimode [ESI and APcI) ionization and an automatic sampler and PDA detector), UV-Spectrometer (190 – 900 nm, double beam line). Collaborations and Projects We have collaborative studies and projects with Ministry of Food and Agriculture, General Directorate of Agricultural Researches and Policies, Istanbul Technical University (Turkey), some leading companies (Turkey). A TÜBİTAK (Scientific and Technological Research Council of Turkey) project aimed at development of a new spectrofluorometric method with resorcinol probe for measurement of hypochlorous acid scavenging activity of polyphenols is ongoing. Research Infrastructure of the Laboratory HPLC (UV-Vis, luorescence and PDA detector and post-column derivatization system), Electrochemical potentiostat/galvanostat, Mercury drop electrode cel- Also, ongoing studies are classification and assessment of food stuffs according to their antioxidant contents, and development of sensors for antioxidant enzyme activity measurement. lular set, Rotary disk electrode cellular set, UPLC/ MS/MS (Mass-Mass triple quadropole) (with ElecLaboratory Supervisor and Contact Information Prof. Dr. Reşat Apak +90 212 4737028 rapak@istanbul.edu.tr FACULTY of ENGINEERING BIOCHEMISTRY RESEARCH LABORATORY O ur laboratory investigates enzyme activity assays and various biochemical parameters assays in animal tissue and serum samples, and enzyme inhibition tests, antioxidant activity assays in various plant extracts and chemicals, enzyme purification, enzyme immobilization, effects of various synthesized inorganic and organic substances on enzyme activities, experimental diabetes, examination of protective effects of various chemicals and plant extracts in experimentally created toxicity models, hyperlipidemia, and determination of contents of plant extracts. Research Infrastructure of the Laboratory HPLC, Spectrophotometer, Spectrofluorometer, Electrophoresis device, Gel imaging device, Frigorific centrifuge, -80°C refrigerator, Freeze dryer, ELISA device. Collaborations and Projects We have collaborative studies and projects with Marmara University (Turkey), Yıldız Technical University (Turkey), Giresun University (Turkey), İnönü University (Turkey) and İstanbul Aydın University (Turkey). Laboratory Supervisor and Contact Information Prof. Dr. Refiye Yanardağ +90 212 4737037 - 17792 yanardag@istanbul.edu.tr 93 FACULTY of ENGINEERING PROCESS and REACTOR DESIGN RESEARCH LABORATORY I ncluded among the activities of our laboratory are production of catalytic hydrogen from natural gas, dehydrogenation of oxidative propane, catalytic reactions such as methane pairing, development of catalysts, such fuel cell technologies as polymer electrolyte fuel cells and solid oxide fuel cells, as well as membrane syntheses, and technologies for preparation of different catalysts. limited high temperature ovens (maximum temperatures 1750°C, 1300°C and 1000°C) and drying ovens, BET Surface Area device, Ultrasound Device (40 kHz). Collaborations and Projects The laboratory took part in the project titled “Electrical Neuronal Oscillations and Cognition” within the scope of the COST program by European Union. A joint project has been conducted with Macedonian Academy of Sciences on EEG-Neurofeedback within 94 the scope of TÜBİTAK’s (Scientific and TechnologResearch Infrastructure of the Laboratory ical Research Council of Turkey) Macedonia-Turkey bilateral research support. Gas chromatography, micro reactor-mass spectrometer combined system, Electrochemical impedance spectroscopy (30 MHz – 1 mHz), Scanning electron microscope (SEM), UV spectrophotometer, Test stations for solid oxide fuel cells and polymer electrolyte fuel cells (PEMFC), Thermogravimetric analysis – differential thermal analysis simultaneous measurement device (maximum temperature 1200°C), Contact angle measurement device, Ultrasound provider, Laboratory Supervisor and Contact Information Prof. Dr. M. Ali Gürkaynak +90 212 4737070- 17661 mag@istanbul.edu.tr FACULTY of ENGINEERING GEOCHRONOLOGICAL SAMPLE PREPARATION and MINERAL SEPARATION LABORATORY O ur laboratory is engaged in application of processes such as mineral separation and enrichment of rock samples for radiochronological analyses, and separation of zircon, xenothyme, amphibole, lawsonite, granitite, monazite, mica, etc. minerals used for age determination, and making them ready for analyses. Research Infrastructure of the Laboratory Magnetic separator, Heavy liquids and minerals flotation – sedimentation systems. Collaborations and Projects We have collaborative studies with Çukurova University (Turkey), Fırat University (Turkey), Istanbul Technical University (Turkey), and Bulgarian Science Academy (Bulgaria), in addition to ongoing TÜBİTAK (Scientific and Technological Research Council of Turkey) projects. Laboratory Supervisor and Contact Information Asst. Prof. Dr. Namık Aysal +90 212 4737070 - 17894 aysal@istanbul.edu.tr 95 FACULTY of FORESTRY SOIL SCIENCE (AGROLOGY) and ECOLOGY LABORATORY O 96 ur laboratory is engaged in pH, electrical conductivity, carbon, nitrogen and other elemental analyses in plant, turf, soil and water samples, and such analyses as texture, calcium carbonate and water holding (retention) capacity in soil, and studies for identification of forest ecosystems. In addition, projects are ongoing on monitoring of forest ecosystems, mapping of forest soils, plant nutrition, rehabilitation of mineral sites, soil pollution, phytoremediation, determination of effects of air pollution on forests. Research Infrastructure of the Laboratory C/N elemental analysis device, ICP-OES, microwave sample granulator, UV spectrometer, Flow injection device, Nitrogen/protein device, Centrifuge device, Frigorific incubator, Stereo microscope, pH-meter and EC-meter. Collaborations and Projects We have collaborative studies and projects with Korean University (Korea), Debrecen University (Hungary) and Istanbul Technical University (Turkey), Dokuz Eylül University (Turkey), Süleyman Demirel University (Turkey) and many other universities, in addition to ongoing joint projects with Forestry Research Institutes of the Ministry of Forest and Hydraulic Works. There are also pending TÜBİTAK (Scientific and Technological Research Council of Turkey) projects pertaining to the quantity of carbon stored in soil and plant masses in forest ecosystems in tandem with climatic changes. Laboratory Supervisor and Contact Information Asst. Prof. Dr. Serdar Akburak +90 212 3382400 - 25298 sakburak@istanbul.edu.tr FACULTY of FORESTRY NANOTECHNOLOGY LABORATORY and THERMAL ANALYSIS LABORATORY I t is the first Nanotechnology Laboratory and Thermal Analysis Laboratory in forest industry field (wood, wooden composite materials, biomaterials, thermoset/thermoplastic glues, furniture) in Turkey. It deals with research and development and innovation studies on applications of nanoscience and nanotechnology in wood industry, and with thermal characterization of materials. Also, ongoing are scientific and industrial projects related to development of new generation of environmental-friendly wooden composites, development of composites with reduced formaldehyde emission, development of composites with enhanced resistance and thermal stability, and development and characterization of biomaterials, biocomposites and nanocomposites. Research Infrastructure of the Laboratory Thermogravimetric Analysis/Differential Thermal Analysis (TGA/DTA) system, Dynamic Mechanical Thermal Analysis/Thermomechanic Analysis (DMTA/TMA) system, Vacuum oven, Ultrasonic homogenizator, Viscosimeter. Collaborations and Projects We have collaborative studies and projects with Maine University (USA), Louisiana State University (USA), Tennessee University (USA), Bangor University (United Kingdom), and Beijing Forestry University (People’s Republic of China), in addition to ongoing joint research and development projects. We also have memberships in European Union COST actions regarding nanotechnology and composite materials. We have also pending joint projects and collaborations with Sabancı University (Turkey), Istanbul Technical University (Turkey) and Bursa Technical University (Turkey) and many other universities and non-governmental organizations. Laboratory Supervisor and Contact Information Prof. Dr. Nusret As +90 212 3382400 - 25361 nusretas@istanbul.edu.tr 97 FACULTY of FORESTRY PROF. DR. A. YILMAZ BOZKURT WOODEN MATERIALS COMBUSTION TEST and ANALYSIS LABORATORY O ur laboratory is engaged in measurement of mass loss and convection speed occurring in wooden materials during combustion, and combustion tests, thermal analyses of wooden materials, and anti-fire performances of wood and wooden-based products and non-wood constructional materials. Research Infrastructure of the Laboratory Mass loss calorimeter, Single-flame source ignitability analyzer, Fourier Transform Infrared Spectrometer (FT-IR). Collaborations and Projects We have collaborative studies and projects with Forestry Service, Forest Products Laboratory (USA) and Yıldız Technical University (Turkey), on various boron products which have not yet been introduced to 98 forestry industry, and on synthesis of boron products as a fire-retardant additive. Laboratory Supervisor and Contact Information Prof. Dr. S. Nami Kartal Research Assistant Evren Terzi +90 212 3382400 - 25352 snkartal@istanbul.edu.tr +90 212 3382400 - 25086 evrent@istanbul.edu.tr FACULTY of FORESTRY ANNUAL RING RESEARCHES and WOOD ANATOMY LABORATORY I t is the first and single laboratory dealing with annual ring research in Turkey. Accordingly, it generates data for various disciplines such as forestry, climate sciences, geography, archeology, history of art, architecture, etc. The laboratory conducts its “wood annual ring” and “wood anatomy” studies together. The history of climate in Anatolia is studied, and annual ring data sets are created for our natural tree species. Furthermore, wood materials found in historical buildings and archeological sites are diagnosed, and their period of use, and building construction and/or repair dates are determined in reliance upon annual ring analyses. In addition, dendrogeomorphological studies are conducted for determination of frequency and limits of such events as avalanche, landslide, etc. Operating as a part of ISTO Herbarium, this laboratory is engaged not only in ecological wood anatomy researches, but also in wood anatomy leg of taxonomic revision studies of wood-like plants, and holds an important collection of contemporary and petrified woods. Research Infrastructure of the Laboratory Linear-tray and computer-aided Annual Ring Measurement Systems, Microscopes, Microtomes, Increment borers and cleaning sets of different sizes. Collaborations and Projects We have collaborative studies and projects on dendrochronology, dendroclimatology, dendroarcheology, dendroecology and dendrogeomorphology with Arizona University (USA), Columbia University (USA) and Purdue University (USA), in addition to collaborative projects ongoing with various local universities such as Istanbul Technical University (Turkey), Ege University (Turkey) and Bartın University (Turkey), and with Forestry Research Institutes. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Nesibe Köse +90 212 3382400 - 25323 nesibe@istanbul.edu.tr 99 FACULTY of FORESTRY PROF. DR. A. YILMAZ BOZKURT WOOD ANATOMY LABORATORY O 100 ur laboratory is engaged in detection of local and exotic tree species in the merchandized raw materials, semi-finished and finished products, and determination of tree species in restoration / conservation projects and in archeological wooden objects, and determination of desired quality specifications of raw material wood at the place of use in tandem with anatomic composition, and identification of material-based problems emerging as a result of anatomic composition and development of resolution suggestions in Forestry Products Industry, and determination of quality losses in wooden materials through examination of anatomic composition, and assessment of the degree of success of industrial processes on woods in line with changes caused thereby in anatomic composition. Furthermore, it covers Prof. Dr. Adnan BERKEL Xylarium (ISTUFw Xylarium), being the first and single collection accepted to International Xylariums (INDEX XYLARIUM) used for education, scientific research, wood diagnosis and industrial service purposes. Research Infrastructure of the Laboratory Stereo microscope, Optical microscope with digital imaging system with polarized and fluorescence equipment and slide microtome. Collaborations and Projects We have collaborative studies and projects with Ministry of Culture and Tourism, Istanbul Restoration and Conservation Central Laboratory Directorate, National Union of Wood, Istanbul Metropolitan Municipality, Protection Application and Supervision Directorate, National Research Institute on Cultural Features (Japan), and Natural Resources and Applied Life Science University (Austria). In addition to a TÜBİTAK (Scientific and Technological Research Council of Turkey) project focused on anatomic and microbiological specifications of archeological wooden materials found in Marmaray Project excavations (Turkey), and effects of thermal process on anatomic composition of wood, we have carried out projects for diagnosis of tree species in archeological wood finds of Aegean Region. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Ayşe Dilek Doğu Research Assistant F. Diğdem Tuncer +90 212 3382400 - 25084 addogu@istanbul.edu.tr +90 212 3382400 - 25351 digdem.kangaloglu@istanbul.edu.tr FACULTY of FORESTRY – FORESTRY PRODUCTS CHEMISTRY and TECHNOLOGY RESEARCH LABORATORY and CELLULOSE, PAPER and CARDBOARD ANALYSIS LABORATORY I ncluded among its activities are clarification of chemical composition of wood-based and non-wood lignocelluloses-based materials, and determination of structures and specifications of new products produced as a result of chemical transformation of these materials, and identification of places of use of them, as well as analyses relating to processes from cellulose production to final area of use of paper, and structural analyses of woods and wood-based materials, as a part of industrial services. Research Infrastructure of the Laboratory HPLC, UV-Vis spectrometer, GC-MS, Fourier Transform Infrared Spectrometer (FT-IR), Viscosimeter, Rheometer, TMA, Universal tester (for paper products), Spectrophotometer (Elrepho), Glossy paper coating device, Manual paper manufacturing devices fit to various different standards. Collaborations and Projects We have collaborative studies and projects with Yıldız Technical University (Turkey), Kastamonu University (Turkey), Gebze High Technology Institute (Turkey), Dokuz Eylül University (Turkey), İzmit Poplar Industry Research Institute (Turkey), Western Michigan University (USA), and North Carolina University (USA), in addition to joint projects with private sector entities, and TÜBİTAK (Scientific and Technological Research Council of Turkey) projects. Laboratory Supervisor and Contact Information Prof. Dr. Celil Atik +90 212 3382400 - 25375 atikc@istanbul.edu.tr 101 FACULTY of FORESTRY BASIN MANAGEMENT LABORATORY I t consists of two separate laboratories where water and soil analyses are conducted. These laboratories offer services on basin, hydrology, hydroecology, soil, etc. research by international methods and through new generation detailed analyses in line with needs and demands of public and private sectors. Research Infrastructure of the Laboratory Kjeldahl semi-automatic nitrogen tester, Centrifuge, Turbidimeter, Ion-meter, Spectrophotometer, pH-meter and EC-meter, Dissolved oxygen tester, water turbidity meter, Soil moisture tester, Permeability device, Penetrometer, Lysimeter. Collaborations and Projects We have collaborative studies and pending projects with Massachusetts University (USA), Department 102 of Agriculture, South Research Station (USA), Çankırı Karatekin University, Süleyman Demirel University (Turkey) and Forestry Research Institutes, focused on basin management, semi-arid areas as a part of climatic changes, pasture improvement. Laboratory Supervisor and Contact Information Prof. Dr. Kamil Şengönül +90 212 3382400 - 25338 sengonul@istanbul.edu.tr FACULTY of AQUACULTURE INSTRUMENTAL ANALYSIS LABORATORY I t deals with quality control, hygiene and sanitation of aquacultural products from fishing to consumption, and aquacultural toxicology, food safety and product development. This laboratory conducts analyses for determination of fatty acid, amino acid, trimethylamine nitrogen and thiobarbituric acid indices, and quantification of 4-hexylresorcinol and biogenic amine, and color measurement and texture measurement analysis in aquacultural products. Research Infrastructure of the Laboratory Gas chromatography, High pressure liquid chromatography, UV-Vis spectrometer, Ultrasonic water bath, Texture measurement device, Color measurement device, Frigorific centrifuge. Collaborations and Projects As a part of TÜBİTAK (Scientific and Technological Research Council of Turkey) supported and other internationally funded projects, various projects focused on protection of aquacultural products and prolongation of their shelf lives have been completed, and a European Union project is continuing for formation of monitoring systems for food safety in aquaculture. Laboratory Supervisor and Contact Information Prof. Dr. Özkan Özden +90 212 4555700 - 16440 ozden@istanbul.edu.tr 103 FACULTY of AQUACULTURE ECOTOXICOLOGY and CYANOTOXIN LABORATORY A ccumulation of cyanotoxins produced by cyanobacteria in aquatic biota, and determination of genes producing cyanotoxins, and effects of environmental factors on cyanotoxin production, and accumulation and convection of heavy metals in food chain, and lethal dose studies for organism groups, and pesticide accumulation in living creatures and water, and PAH analyses are conducted. Research Infrastructure of the Laboratory HPLC, ICP-MS, GS-MS, Real time PCR, Imaging device, Freeze dryer, Cyanobacteria cultivation room, TOC-meter, Laminar flow cabin, Ultrasonicator, Spectrophotometer, Filtration system and microscopes. Collaborations and Projects Three European Union projects are continuing on examination of cyanotoxins in our internal waters 104 and seas, and determination of their effects. Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Reyhan Akçaalan Albay +90 212 4555700 - 16453 akcaalan@istanbul.edu.tr TECHNOLOGY TRANSFER APPLICATION AND RESEARCH CENTER CENTRAL RESEARCH LABORATORY (MERLAB) I t is founded for analyses in line with demands of both Istanbul University organization units and other public and private entities. Clarification of composition of organic ingredients (synthetic organic ingredients, natural organic ingredients, organometric ingredients, organic metal complexes, polymers, etc.), trace element assays, identification of organic ingredients, determination of elemental percentages of solid or liquid organic ingredients by combustion, qualitative and quantitative assay of inorganic complexes and organic ingredients, quantitative and qualitative analyses of elements contained in solid, powder and liquid samples, examination of single crystalline – powder crystalline structures, molecule structures and molecule modeling of small molecules, and qualitative mineral analyses. cm-1, with ATR unit) • X-Ray Powder Crystal Diffraction (Rigaku D, with high temperature oven unit, Cu source, and graphite monochromator) • X-Ray Single Crystal Diffractometer (Rigaku R-Axis Rapid-S) • X-Ray Fluorescence Spectrometer • Liquid Chromatography – Mass Spectrometer • Gas Chromatography (Thermo Finnigan Trace GC Ultra) – Mass Spectrometer (Thermo Finnigan Trace DSQ) • Mass Spectrometer (Thermo Finnigan Trace LCQ) • Ultraviolet Visible Spectrometer (190 – 600 nm) Research Infrastructure of the Laboratory • Atomic Absorption Spectrophotometer (Analyst 200 flame, Analyst 600 Graphite oven and Perkin • Nuclear Magnetic Resonance Spectrometer (Var- Elmer Hydride Systems). ian UNITY INOVA 500 MHz, 1D and 2D NMR, APT, DEPT) • Elemental Analyzer (Thermo Finnigan Flash EA 1112 Series) • Inductive Coupling Plasma – Mass Spectrometer (Thermo Elemental X Series 2) • FT-IR Spectrometer (Perkin Elmer, 450 - 4000 Collaborations and Projects Contemporary method studies are continuing on X-ray diffraction with Max Planck Institute (Germany) and Rikagu/MSC Woodland Texas Laboratory (USA). In addition, contemporary NMR and Elemental Analysis method studies are carried out with Varian Application Laboratory (Germany). Laboratory Supervisor and Contact Information Assoc. Prof. Dr. Hasret Demircan Yardibi +90 212 4400000 - 17772 merlab@istanbul.edu.tr 105 SOCIAL SCIENCES RESEARCH LABORATORIES FACULTY of COMMUNICATION PRODUCTION and POST-PRODUCTION LABORATORY T 108 his laboratory owns all generally accepted professional software programs used for editing, compositing and color grading works for digital videos. It is composed of 6 rooms, four of which are offline editing rooms, one is sound recording room, and one is “Da Vinci Resolve” room used for final mixing. The laboratory has a world standard infrastructure for work flow after a healthy and reliable shooting. It is technically satisfactory, reassuring, quick and creative for users. Research Infrastructure of the Laboratory CMS 65 Reference Monitors, ART TPS II – Microphone Preamp. Collaborations and Projects Montage, cutting, color arrangement and sound mix of short documentary film named Kamilet - 2014 Trento Mountain Films Festival (Italy); montage, cutting, color arrangement and sound mix of medium-length documentary film named Antigoni – Finalist in 2013 Antalya Film Festival; vocal records and mix of new album of famous Rock musician named Umut Kaya; Nursing Faculty Skill Videos; montage and cutting of around 200 skill videos sponsored by Florence Nightingale Foundation; and Tangent Device - DA VINCI Control Interface, montage and cutting of “Democracy” project spon- LYNX HILO - Analog Digital, Digital Analog Sound sored by YEKÜV and handled by a project of Istan- Converter, BON 24 inch Grade 1 Monitor, MAC bul University Scientific Research Projects Unit. OS Workstation-based montage and cutting system, FCP X, AVID, ADOBE, DA VINCI, WINDOWS Workstation-based montage and cutting system, EDIUS and ADOSE, Main Storage Unit (50 TB), SAS Storage Unit (16 TB), data speed above 1 GB per second, Real Time H.264 Converter, FOCAL Laboratory Supervisor and Contact Information Asst. Prof. Dr. İlkay Nişancı +90 212 4400000 - 12749 ilkay.nisanci@istanbul.edu.tr FACULTY of LETTERS PREHISTORY LABORATORY E arthenware, stones, bones and clay findings of different regions and periods are examined and analyzed in typological, technological and functional terms. This laboratory has the most comprehensive archaeological materials archive of Turkey. The archaeological archive, standing as a reference catalogue, contains materials coming from 1645 mounds, ruins and sites, and systematic – layered – samples of around one hundred archaeological sites, and material samples of prehistoric cultures of Near East and Balkans, and West European Paleolithic Era finds, and material samples coming from all obsidian sources in Turkey, Aegean and Mediterranean regions. Collaborations and Projects Connecticut University (USA), Arizona University (USA), Cornell University (USA), Paris University (France), National Natural History Museum (France), National France Scientific Research Center (France), Lyon University (France), Eberhard Karls Tubingen University (Germany), Ludwig-Maximilian University (Germany), Munich Archaeology Museum (Germany), German Archaeology Institute, Bergbau German Mining Museum (Germany), Karlsruhe University (Germany), Cologne University (Germany), Austrian Science Academy (Austria), Vienna University (Austria), New Bulgarian University (Bulgaria), Sozopol Underwater Archaeology Center (Bulgaria), Czech Republic Science Academy (Czech Republic), Prague University (Czech Republic), Natural History Museum (United Kingdom), Groningen University (Netherlands), Athens Clas- Research Infrastructure of the Laboratory sical Studies American School (Greece), Istanbul Technical University (Turkey), Hacettepe Universi- Multi-enlargement metallurgic microscopes, Stereo ty (Turkey), Koç University (Turkey) and Eskişehir microscopes. Anatolian University (Turkey). Laboratory Supervisor and Contact Information Asst. Prof. Dr. Eylem Özdoğan +90 212 4555700 - 15825 eyozdogan@yahoo.com 109 FACULTY of LETTERS SHIP CONSERVATION and RECONSTRUCTION LABORATORY V arious chemical experiments that may be needed in mortar and plaster analyses, ship models, conservation and restoration works are conducted. Underwater artworks are subject to conservation and restoration works. Research Infrastructure of the Laboratory Heating system stainless steel PEG impregnating tank, Fume hoods, Drying oven, Microscopes and ship modeling tools. Collaborations and Projects We conduct joint projects with Byzantine and Post-Byzantine Monuments Research Center (Greece) and Presidency of the Republic of Turkey. 110 Laboratory Supervisor and Contact Information Research Assistant Taner Güler +90 212 4555700 - 16017 tanerg@istanbul.edu.tr FACULTY of LETTERS YENİKAPI SUNKEN WRECKS RESEARCH CENTER LABORATORY C onservation, restoration, three dimensional drawing, photographing and photomosaic works of sunken ships are performed. Research Infrastructure of the Laboratory FaroArm 3D digitizer, Photograph mechanism with paraflash system, Water purification system, Largescale oven and freeze dryer. Collaborations and Projects We conduct joint projects with Byzantine and Post-Byzantine Monuments Research Center (Greece) and Presidency of the Republic of Turkey and Roskilde Museum (Denmark). 111 Laboratory Supervisor and Contact Information Research Assistant Namık Kılıç +90 212 5880100 namik.kilic@istanbul.edu.tr